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Literature summary for 2.5.1.80 extracted from
- Substrate promiscuity of secondary metabolite enzymes: prenylation of hydroxynaphthalenes by fungal indole prenyltransferases (2011), Appl. Microbiol. Biotechnol., 92, 737-748.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds, which can be then tested for their biological activities in drug discovery and development programs | Aspergillus fumigatus |
| synthesis | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds | Aspergillus fumigatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged 7-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1 | Aspergillus fumigatus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Aspergillus fumigatus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | - |
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 1,6-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,6-diol | - |
? | |
| dimethylallyl diphosphate + 1,7-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,7-diol | - |
? | |
| dimethylallyl diphosphate + 1-naphthol | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalen-1-ol | - |
? | |
| dimethylallyl diphosphate + 2,6-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,6-diol | - |
? | |
| dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 3-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 7-[(3-methylbut-2-en-1-yl)oxy]naphthalen-2-ol + 1,6-bis(3-methylbut-2-en-1-yl)naphthalene-2,7-diol | 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol is the major product | ? | |
| dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
| additional information | substrate specificity of 7-DMATS, overview | Aspergillus fumigatus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7-dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
| 7-DMATS | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus fumigatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the DMATS superfamily | Aspergillus fumigatus |
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Release 2023.1 (January 2023)
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