Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tagged protein | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Aspergillus fumigatus | |
0.137 | - |
L-tryptophan | pH 7.5, 37°C | Aspergillus fumigatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight stimulation | Aspergillus fumigatus | |
additional information | addition of divalent cations is not required | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, calculated | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WYG3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + cyclo-L-Trp-Gly | - |
Aspergillus fumigatus | diphosphate + cyclo-L-7-(3-methylbut-2-enyl)-Trp-Gly | 1.8% of the activity with L-tryptophan | ? | |
dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | diphosphate + D-7-(3-methylbut-2-enyl)-tryptophan | 15.5% of the activity with L-tryptophan | ? | |
dimethylallyl diphosphate + L-Trp-Gly | - |
Aspergillus fumigatus | diphosphate + L-7-(3-methylbut-2-enyl)-Trp-Gly | 10.9% of the activity with L-tryptophan | ? | |
dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + L-7-(3-methylbut-2-enyl)-tryptophan | - |
? | |
additional information | no substrate: geranyl diphosphate, L-phenylalanine, L-tyrosine | Aspergillus fumigatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, calculated | Aspergillus fumigatus |