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Literature summary for 2.5.1.80 extracted from

  • Kremer, A.; Li, S.M.
    Potential of a 7-dimethylallyltryptophan synthase as a tool for production of prenylated indole derivatives (2008), Appl. Microbiol. Biotechnol., 79, 951-961.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His-tagged protein Aspergillus fumigatus

General Stability

General Stability Organism
37°C, absence of substrate, 16 h, 80% residual activity Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein Aspergillus fumigatus
54000
-
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein Aspergillus fumigatus
65000
-
gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WYG3 isoform 7-DMATS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 1-methyl-L-tryptophan
-
Aspergillus fumigatus diphosphate + 1-methyl-7-(3-methylbut-2-enyl)-L-tryptophan 35.8% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 11-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 11-methyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan 19.1% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan 89.4% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-bromo-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-bromo-7-(3-methylbut-2-enyl)-DL-tryptophan 7.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-fluoro-L-tryptophan
-
Aspergillus fumigatus diphosphate + 5-fluoro-7-(3-methylbut-2-enyl)-L-tryptophan 10.7% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
Aspergillus fumigatus diphosphate + 5-hydroxy-7-(3-methylbut-2-enyl)-L-tryptophan 26.7% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-methoxy-7-(3-methylbut-2-enyl)-DL-tryptophan 13.3% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan 74.8% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 6-fluoro-7-(3-methylbut-2-enyl)-DL-tryptophan 33.7% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 6-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan 19.8% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 7-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-DL-tryptophan + ? 0.3% of the yield with L-tryptophan ?
dimethylallyl diphosphate + D-tryptophan
-
Aspergillus fumigatus diphosphate + D-7-(3-methylbut-2-enyl)-tryptophan 11.8% of the yield with L-tryptophan ?
dimethylallyl diphosphate + DL-indole-3-lactic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-DL-indole-3-lactic acid 4.3% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-acetic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-acetic acid 10.8% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-butyric acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-butyric acid 9.1% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-propionic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-propionic acid 8.5% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-pyruvic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-pyruvic acid 4.8% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-beta-homotryptophan
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan 28.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-tryptophan
-
Aspergillus fumigatus diphosphate + L-7-(3-methylbut-2-enyl)-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan hydroxamate
-
Aspergillus fumigatus diphosphate + L-7-(gamma,gamma-dimethylallyl)tryptophan hydroxamate 4.6% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-tryptophan methyl ester
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan methyl ester 7.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + N-acetyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan 7.7% of the yield with L-tryptophan ?
dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan i.e. L-abrine Aspergillus fumigatus diphosphate + Nalpha-methyl-L-7-(gamma,gamma-dimethylallyl)tryptophan 82.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + tryptamine
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-tryptamine 7.5% of the yield with L-tryptophan ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
7-DMATS
-
Aspergillus fumigatus