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Literature summary for 2.5.1.78 extracted from

  • Rossi, A.H.; Farias, A.; Fernandez, J.E.; Bonomi, H.R.; Goldbaum, F.A.; Berguer, P.M.
    Brucella spp. lumazine synthase induces a TLR4-mediated protective response against B16 melanoma in mice (2015), PLoS ONE, 10, e0126827.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.78

Application

Application Comment Organism
medicine partially protective effect of enzyme BLS against B16 melanoma in mice generating a protective response at the first stages of tumor growth but not at later stages, therapeutic effect of BLS vaccination Brucella sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of enzyme BLS in Escherichia coli strain BL21 (DE3) Brucella sp.

Protein Variants

Protein Variants Comment Organism
additional information for evaluation of the effectiveness of enzyme BLS as a preventive vaccine, C57BL/6J mice are immunized with BLS or BLS-OVA, subcutaneous inoculation with B16-OVA melanoma, and analysis of the effect induced by BLS in TLR4-expressing B16 melanoma cells. BLS or BLS-OVA induce a significant inhibition of tumor growth, survival is increased, and 50% of mice immunized with the highest dose of BLS do not develop visible tumors Brucella sp.

Organism

Organism UniProt Comment Textmining
Brucella sp. P61711
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme BLS from Escherichia coli strain BL21 (DE3) by dialysis, anion exchange chromatography, and gel filtration Brucella sp.

Subunits

Subunits Comment Organism
decamer
-
 Brucella sp.

Synonyms

Synonyms Comment Organism
BLS
-
 Brucella sp.
lumazine synthase
-
 Brucella sp.

General Information

General Information Comment Organism
physiological function lumazine synthase (BLS) is a highly immunogenic decameric protein which can accept the fusion of foreign proteins at its ten N-termini. These chimeras are very efficient to elicit systemic and oral immunity without adjuvants. BLS signaling via Toll-like receptor 4 (TLR4) regulates innate and adaptive immune responses, inducing dendritic cell maturation and CD8+ T-cell cytotoxicity Brucella sp.