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Literature summary for 2.5.1.78 extracted from

  • Goldbaum, F.A.; Velikovsky, C.A.; Baldi, P.C.; Mörtl, S.; Bacher, A.; Fossati, C.A.
    The 18-kDa cytoplasmic protein of Brucella species - an antigen useful for diagnosis - is a lumazine synthase (1999), J. Med. Microbiol., 48, 833-839.
    View publication on PubMed

Application

Application Comment Organism
medicine this protein constitutes an interesting candidate for serological diagnosis and for the design of specific chemotherapeutic agents, and its polymeric characteristics could provide the basis for the development of an acellular vaccine Brucella sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The recombinant protein is soluble only under reducing conditions, but alkylation with iodoacetamide renders it soluble in non-reducing media Brucella sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Brucella sp. 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 18000, SDS-PAGE Brucella sp.
90000
-
gel filtration Brucella sp.

Organism

Organism UniProt Comment Textmining
Brucella sp. P61711
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brucella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
Brucella sp. 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
?

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE Brucella sp.
More Brucella spp. lumazine synthase arranges in icosahedric capsids similar to those formed by the lumazine synthases of other bacteria Brucella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Brucella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Brucella sp.