Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.78 extracted from

  • Fischer, M.; Haase, I.; Feicht, R.; Richter, G.; Gerhardt, S.; Changeux, J.P.; Huber, R.; Bacher A.
    Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe (2002), Eur. J. Biochem., 269, 519-526.
    View publication on PubMed
Search for more literature for 2.5.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Schizosaccharomyces pombe

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour diffusion method, crystallizes in space group C222(1). The crystals diffract to a resolution of 2.4 A Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
W27F the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Schizosaccharomyces pombe
W27G the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Schizosaccharomyces pombe
W27H the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Schizosaccharomyces pombe
W27I the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Schizosaccharomyces pombe
W27S the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Schizosaccharomyces pombe
W27Y the replacement of tryptophan 27 by aliphatic amino acids substantially reduces the affinity of the enzyme for riboflavin and for the substrate, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
riboflavin
-
 Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme W27F Schizosaccharomyces pombe
0.003
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme W27Y Schizosaccharomyces pombe
0.005
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, wild-type enzyme Schizosaccharomyces pombe
0.065
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme W27F Schizosaccharomyces pombe
0.067
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, wild-type enzyme Schizosaccharomyces pombe
0.086
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme W27Y Schizosaccharomyces pombe
0.137
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme W27I Schizosaccharomyces pombe
0.145
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme W27H Schizosaccharomyces pombe
0.168
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme W27G Schizosaccharomyces pombe
0.187
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme W27S Schizosaccharomyces pombe
0.23
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme W27I Schizosaccharomyces pombe
0.4
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme W27H Schizosaccharomyces pombe
0.43
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme W27G Schizosaccharomyces pombe
0.46
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme W27S Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17188
-
 5 * 17188, calculated from sequence Schizosaccharomyces pombe
17189
-
 5 * 17189, electrospray MS Schizosaccharomyces pombe
87000
-
 sedimentation equilibrium centrifugation Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Schizosaccharomyces pombe
-
 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9UUB1 var. pombe
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Schizosaccharomyces pombe 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 17188, calculated from sequence Schizosaccharomyces pombe
pentamer 5 * 17189, electrospray MS Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Schizosaccharomyces pombe

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.017
-
 riboflavin pH 7.0, 37°C, wild-type enzyme Schizosaccharomyces pombe