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Literature summary for 2.5.1.78 extracted from

  • Klinke, S.; Zylberman, V.; Bonomi, H.R.; Haase, I.; Guimaraes, B.G.; Braden, B.C.; Bacher, A.; Fischer, M.; Goldbaum, F.A.
    Structural and kinetic properties of lumazine synthase isoenzymes in the order Rhizobiales (2007), J. Mol. Biol., 373, 664-680.
    View publication on PubMed
Search for more literature for 2.5.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brucella abortus
expression in Escherichia coli Brucella melitensis
expression in Escherichia coli Mesorhizobium loti

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform RibH1, bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Comparison with structure of isoform RibH2 Brucella melitensis
isoform RibH1, unliganded, to 2.2 A resolution, and bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Comparison with structure of isoform RibH2 Brucella abortus
isoform RibH2, bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione Mesorhizobium loti

Inhibitors

Inhibitors Comment Organism Structure
5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione crystallization data Brucella abortus
5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione crystallization data Brucella melitensis
5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione crystallization data; crystallization data Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH1, pH 7.0, 37°C Mesorhizobium loti
0.004
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C Brucella melitensis
0.015
-
 L-3,4-dihydroxybutan-2-one 4-phosphate isoform RibH1, pH 7.0, 37°C Mesorhizobium loti
0.02
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH2, pH 7.0, 37°C Mesorhizobium loti
0.225
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C Brucella melitensis
0.45
-
 L-3,4-dihydroxybutan-2-one 4-phosphate isoform RibH2, pH 7.0, 37°C Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16800
-
 5 * 16800, calculated, isoform RibH1 Brucella abortus
16800
-
 5 * 16800, calculated, isoform RibH1 Brucella melitensis
17200
-
 5 * 17200, calculated, isoform RibH1 Mesorhizobium loti
17300
-
 10 * 17300, calculated, isoform RibH2 Mesorhizobium loti
75300
-
 isoform RibH1, light scattering experiments Brucella melitensis
80100
-
 light scattering experiments, isoform RibH1 Brucella abortus
85500
-
 isoform RibH1, light scattering experiments Mesorhizobium loti
158500
-
 isoform RinH2, light scattering experiments Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Brucella abortus
-
-
-
Brucella melitensis
-
 isoform RibH1
-
Mesorhizobium loti Q983B0
-
-
Mesorhizobium loti Q986N2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Brucella melitensis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Mesorhizobium loti 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione + L-3,4-dihydroxybutan-2-one 4-phosphate
-
 Mesorhizobium loti 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 17300, calculated, isoform RibH2 Mesorhizobium loti
pentamer 5 * 16800, calculated, isoform RibH1 Brucella abortus
pentamer 5 * 16800, calculated, isoform RibH1 Brucella melitensis
pentamer 5 * 17200, calculated, isoform RibH1 Mesorhizobium loti

Synonyms

Synonyms Comment Organism
6,7-dimethyl-8-ribityllumazine synthase
-
 Brucella abortus
ribH1
-
 Mesorhizobium loti
RibH2
-
 Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Brucella melitensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH2, pH 7.0, 37°C Mesorhizobium loti
0.003
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C Brucella melitensis
0.04
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione isoform RibH1, pH 7.0, 37°C Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Brucella melitensis