Protein Variants | Comment | Organism |
---|---|---|
K127D | activity similar to wild-type | Saccharomyces cerevisiae |
K181H | selective disproportionate decrease in activity toward several substrates | Saccharomyces cerevisiae |
additional information | deletion of 55 amino acids from the C terminus or mutation of both cysteines of the C2H2 Zn finger to alanine leads to inactivity with all of the anticodon stem-loop minihelices tested and with tRNAs | Schizosaccharomyces pombe |
additional information | mutation of the enzyme in its G34 recognition loop region debilitates it differentially for its G34 substrates | Saccharomyces cerevisiae |
T12A | inactive | Schizosaccharomyces pombe |
Y84S | activity similar to wild-type | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + tRNATrpCCA | Schizosaccharomyces pombe | - |
tRNATrpCCA containing N6-(dimethylallyl)adenosine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H3H1 | - |
- |
Saccharomyces cerevisiae | P07884 | - |
- |
Schizosaccharomyces pombe | Q9UT75 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + adenosine 37 in tRNACys | - |
Saccharomyces cerevisiae | N6-(dimethylallyl)adenosine37 in tRNACys + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine 37 in tRNASer | - |
Saccharomyces cerevisiae | N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine 37 in tRNASer | - |
Schizosaccharomyces pombe | N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA | - |
Saccharomyces cerevisiae | N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA | - |
Schizosaccharomyces pombe | N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine 37 in tRNATyr | - |
Saccharomyces cerevisiae | N6-(dimethylallyl)adenosine37 in tRNATyr + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine 37 in tRNATyrGUA | - |
Schizosaccharomyces pombe | N6-(dimethylallyl)adenosine37 in tRNATyrGUA + diphosphate | - |
? | |
dimethylallyl diphosphate + adenosine37 in tRNATrpCCA | - |
Homo sapiens | N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate | - |
? | |
dimethylallyl diphosphate + oligo-RNA | substrates are 17-nt or 19-nt synthetic oligo-RNAs representing anticondon stem-loops as minihelix analogs of tRNAs. Only the anticodon stem-loops with a predicted stem of 6 bp are efficiently modified | Schizosaccharomyces pombe | oligo-RNA containing N6-(dimethylallyl)adenosine + diphosphate | - |
? | |
dimethylallyl diphosphate + tRNATrpCCA | - |
Schizosaccharomyces pombe | tRNATrpCCA containing N6-(dimethylallyl)adenosine + diphosphate | - |
? | |
additional information | anticondon stem-loops with substitutions as in Ser-G36, Ser-G37, and Ser-G36,G38 greatly diminish activity, and Ser-C38 and Ser-U38 are not modified, while Ser-G38 is a substrate | Schizosaccharomyces pombe | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A37 isopentenyltransferase | - |
Schizosaccharomyces pombe |
Mod5 | - |
Saccharomyces cerevisiae |
Mod5 | - |
Schizosaccharomyces pombe |
Tit1 | - |
Schizosaccharomyces pombe |
TRIT1 | - |
Homo sapiens |