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Literature summary for 2.5.1.75 extracted from

  • Lamichhane, T.N.; Blewett, N.H.; Maraia, R.J.
    Plasticity and diversity of tRNA anticodon determinants of substrate recognition by eukaryotic A37 isopentenyltransferases (2011), RNA, 17, 1846-1857.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
K127D activity similar to wild-type Saccharomyces cerevisiae
K181H selective disproportionate decrease in activity toward several substrates Saccharomyces cerevisiae
additional information deletion of 55 amino acids from the C terminus or mutation of both cysteines of the C2H2 Zn finger to alanine leads to inactivity with all of the anticodon stem-loop minihelices tested and with tRNAs Schizosaccharomyces pombe
additional information mutation of the enzyme in its G34 recognition loop region debilitates it differentially for its G34 substrates Saccharomyces cerevisiae
T12A inactive Schizosaccharomyces pombe
Y84S activity similar to wild-type Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + tRNATrpCCA Schizosaccharomyces pombe
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tRNATrpCCA containing N6-(dimethylallyl)adenosine + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H3H1
-
-
Saccharomyces cerevisiae P07884
-
-
Schizosaccharomyces pombe Q9UT75
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + adenosine 37 in tRNACys
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Saccharomyces cerevisiae N6-(dimethylallyl)adenosine37 in tRNACys + diphosphate
-
?
dimethylallyl diphosphate + adenosine 37 in tRNASer
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Saccharomyces cerevisiae N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate
-
?
dimethylallyl diphosphate + adenosine 37 in tRNASer
-
Schizosaccharomyces pombe N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate
-
?
dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA
-
Saccharomyces cerevisiae N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
-
?
dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA
-
Schizosaccharomyces pombe N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
-
?
dimethylallyl diphosphate + adenosine 37 in tRNATyr
-
Saccharomyces cerevisiae N6-(dimethylallyl)adenosine37 in tRNATyr + diphosphate
-
?
dimethylallyl diphosphate + adenosine 37 in tRNATyrGUA
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Schizosaccharomyces pombe N6-(dimethylallyl)adenosine37 in tRNATyrGUA + diphosphate
-
?
dimethylallyl diphosphate + adenosine37 in tRNATrpCCA
-
Homo sapiens N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
-
?
dimethylallyl diphosphate + oligo-RNA substrates are 17-nt or 19-nt synthetic oligo-RNAs representing anticondon stem-loops as minihelix analogs of tRNAs. Only the anticodon stem-loops with a predicted stem of 6 bp are efficiently modified Schizosaccharomyces pombe oligo-RNA containing N6-(dimethylallyl)adenosine + diphosphate
-
?
dimethylallyl diphosphate + tRNATrpCCA
-
Schizosaccharomyces pombe tRNATrpCCA containing N6-(dimethylallyl)adenosine + diphosphate
-
?
additional information anticondon stem-loops with substitutions as in Ser-G36, Ser-G37, and Ser-G36,G38 greatly diminish activity, and Ser-C38 and Ser-U38 are not modified, while Ser-G38 is a substrate Schizosaccharomyces pombe ?
-
?

Synonyms

Synonyms Comment Organism
A37 isopentenyltransferase
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Schizosaccharomyces pombe
Mod5
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Saccharomyces cerevisiae
Mod5
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Schizosaccharomyces pombe
Tit1
-
Schizosaccharomyces pombe
TRIT1
-
Homo sapiens