Protein Variants | Comment | Organism |
---|---|---|
D153A | at least 25% of wild-type activity | Escherichia coli |
D164A | increase in Km for both substrates | Escherichia coli |
D42A | 20fold decrease in kcat | Escherichia coli |
E173A | at least 25% of wild-type activity | Escherichia coli |
E229A | substantial increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations | Escherichia coli |
F84A | at least 25% of wild-type activity | Escherichia coli |
H67F | 25fold increase in Km for dimethylallyl diphosphate | Escherichia coli |
K23A | increase in Km for both substrates | Escherichia coli |
K280A | increase in Km for both substrates | Escherichia coli |
K56A | 15fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations | Escherichia coli |
Q253A | 18fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations | Escherichia coli |
Q282A | increase in both Km and kcat value | Escherichia coli |
R167A | increase in Km for both substrates | Escherichia coli |
R170A | increase in Km for both substrates | Escherichia coli |
R213A | at least 25% of wild-type activity | Escherichia coli |
R217A | increase in Km and decrease in kcat for dimethylallyl diphosphate | Escherichia coli |
R281A | at least 25% of wild-type activity | Escherichia coli |
S178A | at least 25% of wild-type activity | Escherichia coli |
S43A | at least 25% of wild-type activity | Escherichia coli |
T108A | increase in Km for both substrates | Escherichia coli |
T19A | about 600fold decrease in kcat | Escherichia coli |
T24A | increase in Km for both substrates | Escherichia coli |
T275A | at least 25% of wild-type activity | Escherichia coli |
T54A | increase in Km for both substrates | Escherichia coli |
W285A | at least 25% of wild-type activity | Escherichia coli |
Y111F | at least 25% of wild-type activity | Escherichia coli |
Y111S | at least 25% of wild-type activity | Escherichia coli |
Y47F | at least 25% of wild-type activity | Escherichia coli |
Y47S | 100fold decrease in kcat | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
dimethylallyl diphosphate | mutant Y47S, pH 8.0 | Escherichia coli | |
1.5 | - |
seventeen-base RNA oligonucleotide | mutant H67F, pH 8.0 | Escherichia coli | |
2.2 | - |
dimethylallyl diphosphate | wild-type, pH 8.0 | Escherichia coli | |
2.3 | - |
dimethylallyl diphosphate | mutant Q253A, pH 8.0 | Escherichia coli | |
2.8 | - |
seventeen-base RNA oligonucleotide | mutant D42A, pH 8.0 | Escherichia coli | |
2.9 | - |
dimethylallyl diphosphate | mutant D42A, pH 8.0 | Escherichia coli | |
3.2 | - |
seventeen-base RNA oligonucleotide | wild-type, pH 8.0 | Escherichia coli | |
3.5 | - |
dimethylallyl diphosphate | mutant Y47F, pH 8.0 | Escherichia coli | |
4.4 | - |
seventeen-base RNA oligonucleotide | mutant Y47F, pH 8.0 | Escherichia coli | |
7.2 | - |
seventeen-base RNA oligonucleotide | mutant R217A, pH 8.0 | Escherichia coli | |
8.9 | - |
seventeen-base RNA oligonucleotide | mutant Y47S, pH 8.0 | Escherichia coli | |
10 | - |
dimethylallyl diphosphate | mutant K56A, pH 8.0 | Escherichia coli | |
12 | - |
seventeen-base RNA oligonucleotide | mutant T54A, pH 8.0 | Escherichia coli | |
13 | - |
dimethylallyl diphosphate | mutant T54A, pH 8.0 | Escherichia coli | |
16 | - |
seventeen-base RNA oligonucleotide | mutant T108A, pH 8.0 | Escherichia coli | |
22 | - |
dimethylallyl diphosphate | mutant T108A, pH 8.0 | Escherichia coli | |
27 | - |
dimethylallyl diphosphate | mutant d164A, pH 8.0 | Escherichia coli | |
27 | - |
seventeen-base RNA oligonucleotide | mutant d164A, pH 8.0 | Escherichia coli | |
45 | - |
seventeen-base RNA oligonucleotide | mutant Q282A, pH 8.0 | Escherichia coli | |
50 | - |
dimethylallyl diphosphate | mutant H67F, pH 8.0 | Escherichia coli | |
50 | - |
seventeen-base RNA oligonucleotide | mutant K56A, pH 8.0 | Escherichia coli | |
56 | - |
seventeen-base RNA oligonucleotide | mutant Q253A, pH 8.0 | Escherichia coli | |
60 | - |
dimethylallyl diphosphate | mutant Q282A, pH 8.0 | Escherichia coli | |
75 | - |
seventeen-base RNA oligonucleotide | mutant R167A, pH 8.0 | Escherichia coli | |
114 | - |
dimethylallyl diphosphate | mutant R217A, pH 8.0 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + seventeen-base RNA oligonucleotide | sequence 5'GCGGACUCAAAAUCCGC3', RNA oligonucleotide based on the unmodified stem-loop region of tRNAPhe | Escherichia coli | diphosphate + seventeen-base RNA oligonucleotide containing 6-dimethylallyladenosine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
seventeen-base RNA oligonucleotide | mutant Y47S, pH 8.0 | Escherichia coli | |
0.007 | - |
seventeen-base RNA oligonucleotide | mutant R217A, pH 8.0 | Escherichia coli | |
0.009 | - |
seventeen-base RNA oligonucleotide | mutant D42A, pH 8.0 | Escherichia coli | |
0.07 | - |
seventeen-base RNA oligonucleotide | mutant T108A, pH 8.0 | Escherichia coli | |
0.07 | - |
seventeen-base RNA oligonucleotide | mutant T54A, pH 8.0 | Escherichia coli | |
0.15 | - |
seventeen-base RNA oligonucleotide | mutant D164A, pH 8.0 | Escherichia coli | |
0.18 | - |
seventeen-base RNA oligonucleotide | mutant H67F, pH 8.0 | Escherichia coli | |
0.19 | - |
seventeen-base RNA oligonucleotide | wild-type, pH 8.0 | Escherichia coli | |
0.23 | - |
seventeen-base RNA oligonucleotide | mutant Y47F, pH 8.0 | Escherichia coli | |
0.3 | - |
seventeen-base RNA oligonucleotide | mutnat Q282A, pH 8.0 | Escherichia coli |