Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, N-terminal His-tag | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C113A | 1 mol of iron and sulfur per mol of protein, oligomer formation | Escherichia coli |
C119A | 2.9 mol of iron and sulfur per mol of protein | Escherichia coli |
C200A | 0.9 mol of iron and sulfur per mol of protein, oligomer formation | Escherichia coli |
C291A | 3.9 mol of iron and sulfur per mol of protein | Escherichia coli |
C291A/C294A | 3.7 mol of iron and sulfur per mol of protein | Escherichia coli |
C291A/C294A/C297A | 0.5 mol of iron and sulfur per mol of protein | Escherichia coli |
C294A | 3.2 mol of iron and sulfur per mol of protein | Escherichia coli |
C294A/C297A | 0.6 mol of iron and sulfur per mol of protein | Escherichia coli |
C297A | 0.4 mol of iron and sulfur per mol of protein, oligomer formation | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | protein contains 4 mol of iron and sulfur per mol | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39300 | - |
1 * 40000, SDS-PAGE, 1 * 39300, calculated | Escherichia coli |
40000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE, 1 * 39300, calculated | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | protein contains 4 mol of iron and sulfur per mol | Escherichia coli |