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Literature summary for 2.5.1.72 extracted from

  • Rousset, C.; Fontecave, M.; Ollagnier de Choudens, S.
    The [4Fe-4S] cluster of quinolinate synthase from Escherichia coli: investigation of cluster ligands (2008), FEBS Lett., 582, 2937-2944.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, N-terminal His-tag Escherichia coli

Protein Variants

Protein Variants Comment Organism
C113A 1 mol of iron and sulfur per mol of protein, oligomer formation Escherichia coli
C119A 2.9 mol of iron and sulfur per mol of protein Escherichia coli
C200A 0.9 mol of iron and sulfur per mol of protein, oligomer formation Escherichia coli
C291A 3.9 mol of iron and sulfur per mol of protein Escherichia coli
C291A/C294A 3.7 mol of iron and sulfur per mol of protein Escherichia coli
C291A/C294A/C297A 0.5 mol of iron and sulfur per mol of protein Escherichia coli
C294A 3.2 mol of iron and sulfur per mol of protein Escherichia coli
C294A/C297A 0.6 mol of iron and sulfur per mol of protein Escherichia coli
C297A 0.4 mol of iron and sulfur per mol of protein, oligomer formation Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein contains 4 mol of iron and sulfur per mol Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39300
-
1 * 40000, SDS-PAGE, 1 * 39300, calculated Escherichia coli
40000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Escherichia coli

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE, 1 * 39300, calculated Escherichia coli

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre protein contains 4 mol of iron and sulfur per mol Escherichia coli