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Literature summary for 2.5.1.72 extracted from

  • Saunders, A.H.; Griffiths, A.E.; Lee, K.H.; Cicchillo, R.M.; Tu, L.; Stromberg, J.A.; Krebs, C.; Booker, S.J.
    Characterization of quinolinate synthases from Escherichia coli, Mycobacterium tuberculosis, and Pyrococcus horikoshii indicates that [4Fe-4S] clusters are common cofactors throughout this class of enzymes (2008), Biochemistry, 47, 10999-11012.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
-
 Mycobacterium tuberculosis
-
 Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
C113A 0.8 iron ions per polypeptide, no catalytic activity Escherichia coli
C113S 1.3 iron ions per polypeptide, no catalytic activity Escherichia coli
C119S 1.0 iron ions per polypeptide Escherichia coli
C128S 2.7 iron ions per polypeptide Escherichia coli
C195S 1.5 iron ions per polypeptide Escherichia coli
C200A 1.5 iron ions per polypeptide, no catalytic activity Escherichia coli
C200S 1.0 iron ions per polypeptide, no catalytic activity Escherichia coli
C291S 0.8 iron ions per polypeptide Escherichia coli
C294S 2.1 iron ions per polypeptide Escherichia coli
C297A 0.3 iron ions per polypeptide, no catalytic activity Escherichia coli
C297S 0.3 iron ions per polypeptide, no catalytic activity Escherichia coli
C64S 1.4 iron ions per polypeptide Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein contains a [4Fe-4S] cluster absolutely required for activity Mycobacterium tuberculosis
Iron protein contains a [4Fe-4S] cluster absolutely required for activity Pyrococcus horikoshii
Iron protein contains a [4Fe-4S] cluster absolutely required for activity. 3 iron ions per polypeptide Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis
-
-
-
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Mycobacterium tuberculosis
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + iminoaspartate
-
 Escherichia coli ? + H2O + phosphate
-
 ?
dihydroxyacetone phosphate + iminoaspartate
-
 Mycobacterium tuberculosis ? + H2O + phosphate
-
 ?
dihydroxyacetone phosphate + iminoaspartate
-
 Pyrococcus horikoshii ? + H2O + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NadA
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre protein contains a [4Fe-4S] cluster absolutely required for activity Escherichia coli
iron-sulfur centre protein contains a [4Fe-4S] cluster absolutely required for activity Mycobacterium tuberculosis
iron-sulfur centre protein contains a [4Fe-4S] cluster absolutely required for activity Pyrococcus horikoshii