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Literature summary for 2.5.1.70 extracted from

  • Sasaki, K.; Tsurumaru, Y.; Yamamoto, H.; Yazaki, K.
    Molecular characterization of a membrane-bound prenyltransferase specific for isoflavone from Sophora flavescens (2011), J. Biol. Chem., 286, 24125-24134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Sophora flavescens

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric enzymes between narigenin 8-dimethylallyltransferase and isoflavone-specific prenyltransferase G6DT responsible for the prenylation of the genistein at the 6-position, by recombination of amino acid sequences within the loop domains. Chimera SfG2N possesses purely narigenin 8-dimethylallyltransferase activity despite the absence of the N-terminal sequence derived from narigenin 8-dimethylallyltransferase, 243 amino acids. Chimera SfG3N possesses only isoflavone-specific prenyltransferase G6DT activity, although its 33% of the C-terminus is from narigenin 8-dimethylallyltransferase polypeptide. Neither chimeric enzyme can catalyze the prenylation reaction for other types of flavonoid such as kaempferol, apigenin, naringenin chalcone Sophora flavescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
3,3-dimethylallyl diphosphate pH 9.0, 30°C Sophora flavescens
0.05
-
(2S)-naringenin pH 9.0, 30°C Sophora flavescens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Sophora flavescens 16020
-

Organism

Organism UniProt Comment Textmining
Sophora flavescens F8WPJ0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3-dimethylallyl diphosphate + (2S)-liquiritigenin
-
Sophora flavescens diphosphate + isobavachin
-
?
3,3-dimethylallyl diphosphate + (2S)-naringenin
-
Sophora flavescens diphosphate + sophoraflavanone B
-
?

Synonyms

Synonyms Comment Organism
N8DT-3
-
Sophora flavescens