KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
(2S)-naringenin | pH 9.0, 25°C, microsome | Sophora flavescens | |
0.12 | - |
3,3-dimethylallyl diphosphate | pH 9.0, 25°C, microsome | Sophora flavescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sophora flavescens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 6% of the activity with Mg2+ | Sophora flavescens | |
Co2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
Cu2+ | 1% of the activity with Mg2+ | Sophora flavescens | |
Mg2+ | required, best divalent cation, optimal at 10 mM | Sophora flavescens | |
Mn2+ | 32% of the activity with Mg2+ | Sophora flavescens | |
Zn2+ | 2% of the activity with Mg2+ | Sophora flavescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3-dimethylallyl diphosphate + (2S)-naringenin | Sophora flavescens | two possible pathways leading to sophoraflavanone G, overview | diphosphate + sophoraflavanone B | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sophora flavescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by microsome preparation | Sophora flavescens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3-dimethylallyl diphosphate + (2S)-liquiritigenin | i.e. 4',7-dihydroxyflavanone | Sophora flavescens | diphosphate + isobavachin | - |
? | |
3,3-dimethylallyl diphosphate + (2S)-naringenin | two possible pathways leading to sophoraflavanone G, overview | Sophora flavescens | diphosphate + sophoraflavanone B | - |
? | |
3,3-dimethylallyl diphosphate + (2S)-naringenin | best substrate, the enzyme is enantiospecific for (2S)-naringenin, i.e. (S)-4',5,7-trihydroxy-flavanone, and utilizes 3,3-dimethylallyl diphosphate as sole prenyl donor | Sophora flavescens | diphosphate + sophoraflavanone B | - |
? | |
3,3-dimethylallyl diphosphate + 2'-hydroxynaringenin | with 2'-hydroxynaringenin, i.e. (S)-2',4',5,7-tetrahydroxy-flavanone, the enzyme shows 3% of the activity with naringenin | Sophora flavescens | diphosphate + leachianone G | - |
? | |
3,3-dimethylallyl diphosphate + hesperetin | i.e. 5,7,3'-trihydroxy-4'-methoxyflavanone | Sophora flavescens | diphosphate + (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-8-(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one | - |
? | |
3,3-dimethylallyl diphosphate + taxifolin | i.e. 3,5,7,3',4'-pentahydroxyflavanone | Sophora flavescens | diphosphate + (2R,3R)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-8-(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one | - |
? | |
additional information | substrate specificity, formation of 8-dimethylallyl-, 8-lavandulyl- and 6-dimethylallylflavanones, overview | Sophora flavescens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavanone 8-dimethylallyltransferase | - |
Sophora flavescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sophora flavescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Sophora flavescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | - |
Sophora flavescens |