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Literature summary for 2.5.1.7 extracted from

  • Eschenburg, S.; Priestman, M.; Schoenbrunn, E.
    Evidence that the fosfomycin target Cys115 in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is essential for product release (2005), J. Biol. Chem., 280, 3757-3763.
    View publication on PubMed
Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli STBL2-DE3 cells Enterobacter cloacae

Crystallization (Commentary)

Crystallization (Comment) Organism
at 19°C, from 10 mM MES, pH 6.4, 10% (w/v) polyethylene glycol 20000, in the presence of 5 mM UDP-N-acetyl-D-glucosamine and 5 mM phosphoenolpyruvate Enterobacter cloacae

Protein Variants

Protein Variants Comment Organism
C115S mutant enzyme is capable of catalyzing the wild type enzyme reaction but is unable to release the products (single turnover), activity is not affected by fosfomycin Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
fosfomycin 0.01 mM Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae P33038
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 r

Synonyms

Synonyms Comment Organism
MurA
-
 Enterobacter cloacae
UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Enterobacter cloacae