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Literature summary for 2.5.1.7 extracted from

  • Gunetileke, K.G.; Anwar, R.A.
    Biosynthesis of uridine diphospho-N-acetylmuramic acid. II. Purification and properties of pyruvate-uridine diphospho-N-acetylglucosamine transferase and characterization of uridine diphospho-N-acetylenopyruvylglucosamine (1968), J. Biol. Chem., 243, 5770-5778.
    View publication on PubMed
Search for more literature for 2.5.1.7

Activating Compound

Activating Compound Comment Organism Structure
thiol groups required for activity Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak Enterobacter cloacae
Hg2+ weak Enterobacter cloacae
iodoacetate
-
 Enterobacter cloacae
N-ethylmaleimide
-
 Enterobacter cloacae
p-chloromercuribenzoate
-
 Enterobacter cloacae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 phosphoenolpyruvate
-
 Enterobacter cloacae
0.46
-
 UDP-GlcNAc
-
 Enterobacter cloacae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by K+, Na+, Mg2+, and Mn2+ Enterobacter cloacae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Enterobacter cloacae pathway for biosynthesis of UDP-N-acetylmuramic acid phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Enterobacter cloacae NRC 492 pathway for biosynthesis of UDP-N-acetylmuramic acid phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
-
-
Enterobacter cloacae NRC 492
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
 purified enzyme Enterobacter cloacae

Storage Stability

Storage Stability Organism
0°C or -20°C, 0.2 mg/ml DTT, 40% loss of activity after 2 weeks Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine specific for phosphoenolpyruvate and UDP-N-acetyl-D-glucosamine Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate r
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine pathway for biosynthesis of UDP-N-acetylmuramic acid Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine specific for phosphoenolpyruvate and UDP-N-acetyl-D-glucosamine Enterobacter cloacae NRC 492 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate r
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine pathway for biosynthesis of UDP-N-acetylmuramic acid Enterobacter cloacae NRC 492 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Enterobacter cloacae
42
-
-
 Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7.4
-
 Enterobacter cloacae