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Literature summary for 2.5.1.68 extracted from

  • Wang, W.; Dong, C.; McNeil, M.; Kaur, D.; Mahapatra, S.; Crick, D.C.; Naismith, J.H.
    The structural basis of chain length control in Rv1086 (2008), J. Mol. Biol., 381, 129-140.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.68

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization using sparse matrix screens and vapor diffusion, structure of apo Rv1086 with citronellyl diphosphate bound and with the product mimic (2E,6E)-farnesyl diphosphate bound. The maximal reaction rate of the wild-type enzyme is 10fold greater than that of the mutant enzyme Mycobacterium tuberculosis
in complex with citronellyl diphosphate and with product mimic (E,E)-farnesyl diphosphate Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
L84A potentially important in limiting chain length. The mutant L84A is not expressed in a soluble form Mycobacterium tuberculosis
L84A/L85F mutant enzyme has a Km-value for geranyl diphosphate of 0.15 mM compared to 0.038 mM for wild-type Rv108612 Mycobacterium tuberculosis
L84A/L85F well expressed and active. Mutant protein is capable of synthesizing prenyl diphosphates containing 20 carbon atoms when geranyl diphosphate is used as the allylic substrate and 50 carbon atoms when EE-farnesyl diphosphate is used as the allylic substrate Mycobacterium tuberculosis
V107G inactive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 geranyl diphosphate mutant L84A/L85F Mycobacterium tuberculosis
0.15
-
 geranyl diphosphate pH 7.9, 37°C, mutant enzyme L84A/L85F Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WFF5
-
-
Mycobacterium tuberculosis H37Rv P9WFF5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis ? + diphosphate no substrate for wild-type enzyme. Mutant L84A/L85A synthesizes prenyl diphosphates containing 50 carbon atoms ?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis H37Rv ? + diphosphate no substrate for wild-type enzyme. Mutant L84A/L85A synthesizes prenyl diphosphates containing 50 carbon atoms ?
geranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis (2Z,6E)-farnesyl diphosphate + diphosphate product of wild-type.Mutant L84A/L85A synthesizes prenyl diphosphates containing 20 carbon atoms ?
geranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis H37Rv (2Z,6E)-farnesyl diphosphate + diphosphate product of wild-type.Mutant L84A/L85A synthesizes prenyl diphosphates containing 20 carbon atoms ?
geranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis diphosphate + (2Z,6E)-farnesyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate
-
 Mycobacterium tuberculosis H37Rv diphosphate + (2Z,6E)-farnesyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration and crystallization data Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
Rv1086
-
 Mycobacterium tuberculosis