Literature summary for 2.5.1.68 extracted from

Ambo, T.; Noike, M.; Kurokawa, H.; Koyama, T.
Cloning and functional analysis of novel short-chain cis-prenyltransferases (2008), Biochem. Biophys. Res. Commun., 375, 536-540.

Search for more literature for 2.5.1.68

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mycobacterium tuberculosis
expression in Escherichia coli	Corynebacterium glutamicum
expression in Escherichia coli	Thermobifida fusca
expression in Escherichia coli	Corynebacterium efficiens

Protein Variants

Protein Variants	Comment	Organism
I59A	change in product chain-length, produces prenyl products with chain length up to C50	Thermobifida fusca
I59F	inactive mutant enzyme	Thermobifida fusca
I59M	change in product chain-length, produces prenyl products with chain length up to C50	Thermobifida fusca
I59V	change in product chain-length, produces prenyl products with chain length up to C50	Thermobifida fusca

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	isopentenyl diphosphate	pH 7.5, 37°C	Thermobifida fusca
0.0023	-	geranyl diphosphate	pH 7.5, 37°C	Thermobifida fusca
0.0027	-	isopentenyl diphosphate	pH 7.5, 37°C	Corynebacterium efficiens
0.0041	-	geranyl diphosphate	pH 7.5, 37°C	Corynebacterium efficiens
0.0045	-	isopentenyl diphosphate	pH 7.5, 37°C	Mycobacterium tuberculosis
0.0067	-	isopentenyl diphosphate	pH 7.5, 37°C	Corynebacterium glutamicum
0.0068	-	geranyl diphosphate	pH 7.5, 37°C	Corynebacterium glutamicum
0.0078	-	geranyl diphosphate	pH 7.5, 37°C	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, SDS-PAGE, calculated from sequence	Corynebacterium glutamicum
31000	-	x * 31000, SDS-PAGE, calculated from sequence	Thermobifida fusca
31000	-	x * 31000, SDS-PAGE, calculated from sequence	Corynebacterium efficiens

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium efficiens	-	-	-
Corynebacterium glutamicum	-	-	-
Mycobacterium tuberculosis	-	-	-
Thermobifida fusca	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Corynebacterium glutamicum
-	Thermobifida fusca
-	Corynebacterium efficiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate + isopentenyl diphosphate	only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate	Corynebacterium glutamicum	diphosphate + (2Z,6E)-farnesyl diphosphate	-	?
geranyl diphosphate + isopentenyl diphosphate	only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate	Mycobacterium tuberculosis	diphosphate + (2Z,6E)-farnesyl diphosphate	-	?
geranyl diphosphate + isopentenyl diphosphate	only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate	Thermobifida fusca	diphosphate + (2Z,6E)-farnesyl diphosphate	-	?
geranyl diphosphate + isopentenyl diphosphate	only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate	Corynebacterium efficiens	diphosphate + (2Z,6E)-farnesyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, SDS-PAGE, calculated from sequence	Corynebacterium glutamicum
?	x * 31000, SDS-PAGE, calculated from sequence	Thermobifida fusca
?	x * 31000, SDS-PAGE, calculated from sequence	Corynebacterium efficiens

Synonyms

Synonyms	Comment	Organism
cis-farnesyl diphosphate synthase	-	Corynebacterium glutamicum
cis-farnesyl diphosphate synthase	-	Mycobacterium tuberculosis
cis-farnesyl diphosphate synthase	-	Thermobifida fusca
cis-farnesyl diphosphate synthase	-	Corynebacterium efficiens
cis-FPS	-	Corynebacterium glutamicum
cis-FPS	-	Mycobacterium tuberculosis
cis-FPS	-	Thermobifida fusca
cis-FPS	-	Corynebacterium efficiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Corynebacterium glutamicum
37	-	assay at	Mycobacterium tuberculosis
37	-	assay at	Thermobifida fusca
37	-	assay at	Corynebacterium efficiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
141	-	geranyl diphosphate	pH 7.5, 37°C	Corynebacterium efficiens
173	-	isopentenyl diphosphate	pH 7.5, 37°C	Corynebacterium efficiens
229	-	geranyl diphosphate	pH 7.5, 37°C	Thermobifida fusca
282	-	isopentenyl diphosphate	pH 7.5, 37°C	Thermobifida fusca
329	-	isopentenyl diphosphate	pH 7.5, 37°C	Corynebacterium glutamicum
329	-	geranyl diphosphate	pH 7.5, 37°C	Corynebacterium glutamicum
788	-	isopentenyl diphosphate	pH 7.5, 37°C	Mycobacterium tuberculosis
860	-	geranyl diphosphate	pH 7.5, 37°C	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Corynebacterium glutamicum
7.5	-	assay at	Mycobacterium tuberculosis
7.5	-	assay at	Thermobifida fusca
7.5	-	assay at	Corynebacterium efficiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
34390	-	geranyl diphosphate	pH 7.5, 37°C	Corynebacterium efficiens
48380	-	geranyl diphosphate	pH 7.5, 37°C	Corynebacterium glutamicum
49100	-	isopentenyl diphosphate	pH 7.5, 37°C	Corynebacterium glutamicum
64070	-	isopentenyl diphosphate	pH 7.5, 37°C	Corynebacterium efficiens
99570	-	geranyl diphosphate	pH 7.5, 37°C	Thermobifida fusca
110300	-	geranyl diphosphate	pH 7.5, 37°C	Mycobacterium tuberculosis
175100	-	isopentenyl diphosphate	pH 7.5, 37°C	Mycobacterium tuberculosis
235000	-	isopentenyl diphosphate	pH 7.5, 37°C	Thermobifida fusca

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Release 2023.1 (January 2023)