Cloned (Comment) | Organism |
---|---|
gene flA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) pLysS | Streptomyces cattleya |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the purified recombinant enzyme on poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (2:1) polymer monoliths, method development to imporve immobilization yields and enzyme activity, determination of stability of the immobilized enzyme and enzyme leakage, overview. The enzyme shows over 80% activity during 4 reaction cycles, and 20% activity remaining after the 6th cycle | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya NBRC14057 | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | F8JPG4 | gene flA | - |
Streptomyces cattleya NBRC14057 | F8JPG4 | gene flA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) pLysS by nickel affinity chromatography and gel filtration | Streptomyces cattleya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya NBRC14057 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fluorinase | - |
Streptomyces cattleya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Streptomyces cattleya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces cattleya |