Literature summary for 2.5.1.63 extracted from

Thompson, S.; Onega, M.; Ashworth, S.; Fleming, I.N.; Passchier, J.; OHagan, D.
A two-step fluorinase enzyme mediated (18)F labelling of an RGD peptide for positron emission tomography (2015), Chem. Commun. (Camb.), 51, 13542-13545.

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Application

Application	Comment	Organism
pharmacology	a two-step radiolabelling protocol of a cancer relevant cRGD peptide is described where the fluorinase enzyme is used to catalyse a transhalogenation reaction to generate [18F]-5'-fluoro-5'-deoxy-2-ethynyladenosine, followed by a click reaction to an azide tethered cRGD peptide. This protocol offers efficient radiolabelling of a biologically relevant peptide construct in water at pH 7.8, 37°C in 2 hours, which is metabolically stable in rats and retains high affinity for alphavbeta3 integrin	Streptomyces cattleya
synthesis	a two-step radiolabelling protocol of a cancer relevant cRGD peptide is described where the fluorinase enzyme is used to catalyse a transhalogenation reaction to generate [18F]-5'-fluoro-5'-deoxy-2-ethynyladenosine, followed by a click reaction to an azide tethered cRGD peptide. This protocol offers efficient radiolabelling of a biologically relevant peptide construct in water at pH 7.8, 37°C in 2 hours, which is metabolically stable in rats and retains high affinity for alphavbeta3 integrin	Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + fluoride	Streptomyces cattleya	-	5'-deoxy-5'-fluoroadenosine + L-methionine	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces cattleya	Q70GK9	gene flA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the fluorinase catalyzes a transhalogenation of 5'-chlorodeoxy-2-ethynyladenosine to 5'-fluorodeoxy-2-ethynyladenosinesubstrate specificity. A two-step radiolabelling protocol of a cancer relevant cRGD peptide is described where the fluorinase enzyme is used to catalyse a transhalogenation reaction to generate [18F]-5'-fluoro-5'-deoxy-2-ethynyladenosine, followed by a radiolabelling reaction with an azide tethered cRGD peptide	Streptomyces cattleya	?	-	?
S-adenosyl-L-methionine + fluoride	-	Streptomyces cattleya	5'-deoxy-5'-fluoroadenosine + L-methionine	-	?

Synonyms

Synonyms	Comment	Organism
fluorinase	-	Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Streptomyces cattleya

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Streptomyces cattleya

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Release 2023.1 (January 2023)