Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli C43 (DE3) cells | Streptomyces cattleya |
Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces cattleya |
Protein Variants | Comment | Organism |
---|---|---|
D16A | inactive | Streptomyces cattleya |
D16N | 3% activity compared to the wild type enzyme | Streptomyces cattleya |
D16S | inactive | Streptomyces cattleya |
F156A | 3% activity compared to the wild type enzyme | Streptomyces cattleya |
F156V | 25% activity compared to the wild type enzyme | Streptomyces cattleya |
S158A | 38% activity compared to the wild type enzyme | Streptomyces cattleya |
S158G | 8% activity compared to the wild type enzyme | Streptomyces cattleya |
T80A | 15% activity compared to the wild type enzyme | Streptomyces cattleya |
T80S | 95% activity compared to the wild type enzyme | Streptomyces cattleya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
S-adenosyl-L-methionine | mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.003 | - |
S-adenosyl-L-methionine | mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.0047 | - |
S-adenosyl-L-methionine | mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.0065 | - |
S-adenosyl-L-methionine | wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.0092 | - |
S-adenosyl-L-methionine | mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
1.36 | - |
fluoride | mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
5.4 | - |
fluoride | mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
10.2 | - |
fluoride | wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
18.4 | - |
fluoride | mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
36.8 | - |
fluoride | mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-fluoro-5'-deoxy adenosine synthetase | - |
Streptomyces cattleya |
FDAS | - |
Streptomyces cattleya |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000012 | - |
fluoride | mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.000013 | - |
fluoride | mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.000067 | - |
fluoride | mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.000083 | - |
S-adenosyl-L-methionine | mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.0001 | - |
fluoride | mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.00015 | - |
S-adenosyl-L-methionine | mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.001 | - |
S-adenosyl-L-methionine | mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.001 | - |
fluoride | mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.001 | - |
fluoride | wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya | |
0.0012 | - |
S-adenosyl-L-methionine | wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C | Streptomyces cattleya |