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Literature summary for 2.5.1.63 extracted from

  • Zhu, X.; Robinson, D.A.; McEwan, A.R.; OHagan, D.; Naismith, J.H.
    Mechanism of enzymatic fluorination in Streptomyces cattleya (2007), J. Am. Chem. Soc., 129, 14597-14604.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes expressed in Escherichia coli C43 (DE3) cells Streptomyces cattleya

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Streptomyces cattleya

Protein Variants

Protein Variants Comment Organism
D16A inactive Streptomyces cattleya
D16N 3% activity compared to the wild type enzyme Streptomyces cattleya
D16S inactive Streptomyces cattleya
F156A 3% activity compared to the wild type enzyme Streptomyces cattleya
F156V 25% activity compared to the wild type enzyme Streptomyces cattleya
S158A 38% activity compared to the wild type enzyme Streptomyces cattleya
S158G 8% activity compared to the wild type enzyme Streptomyces cattleya
T80A 15% activity compared to the wild type enzyme Streptomyces cattleya
T80S 95% activity compared to the wild type enzyme Streptomyces cattleya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
S-adenosyl-L-methionine mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.003
-
S-adenosyl-L-methionine mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.0047
-
S-adenosyl-L-methionine mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.0065
-
S-adenosyl-L-methionine wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.0092
-
S-adenosyl-L-methionine mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
1.36
-
fluoride mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
5.4
-
fluoride mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
10.2
-
fluoride wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
18.4
-
fluoride mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
36.8
-
fluoride mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + fluoride
-
Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?

Synonyms

Synonyms Comment Organism
5'-fluoro-5'-deoxy adenosine synthetase
-
Streptomyces cattleya
FDAS
-
Streptomyces cattleya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000012
-
fluoride mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.000013
-
fluoride mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.000067
-
fluoride mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.000083
-
S-adenosyl-L-methionine mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.0001
-
fluoride mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.00015
-
S-adenosyl-L-methionine mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.001
-
S-adenosyl-L-methionine mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.001
-
fluoride mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.001
-
fluoride wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
0.0012
-
S-adenosyl-L-methionine wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya