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Literature summary for 2.5.1.63 extracted from

  • Cadicamo, C.D.; Courtieu, J.; Deng, H.; Meddour, A.; O'Hagan, D.
    Enzymatic fluorination in Streptomyces cattleya takes place with an inversion of configuration consistent with an SN2 reaction mechanism (2004), Chembiochem, 5, 685-690.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
K+ in the coupled assay with SAM synthase and fluorinase Streptomyces cattleya
Mg2+ in the coupled assay with SAM synthase and fluorinase Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
5'-deoxy-5'-fluoroadenosine + L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine reaction mechanism, inversion of configuration consistent with a SN2 reaction mechanism Streptomyces cattleya

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
development of a coupled assay with SAM synthase and fluorinase Streptomyces cattleya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + fluoride
-
Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?
S-adenosyl-L-methionine + fluoride reaction mechanism analysis utilizing a stereospecifically labelled substrate (5'R)-[5'-2H]-ATP in a coupled assay, inversion of configuration consistent with a SN2 reaction mechanism Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine i.e. 5'-FDA, NMR product analysis ?

Synonyms

Synonyms Comment Organism
5'-FDA synthase
-
Streptomyces cattleya
5'-FDAS
-
Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces cattleya