Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | in the coupled assay with SAM synthase and fluorinase | Streptomyces cattleya | |
Mg2+ | in the coupled assay with SAM synthase and fluorinase | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine | reaction mechanism, inversion of configuration consistent with a SN2 reaction mechanism | Streptomyces cattleya |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of a coupled assay with SAM synthase and fluorinase | Streptomyces cattleya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | reaction mechanism analysis utilizing a stereospecifically labelled substrate (5'R)-[5'-2H]-ATP in a coupled assay, inversion of configuration consistent with a SN2 reaction mechanism | Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | i.e. 5'-FDA, NMR product analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
5'-FDA synthase | - |
Streptomyces cattleya |
5'-FDAS | - |
Streptomyces cattleya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces cattleya |