Cloned (Comment) | Organism |
---|---|
gene chlG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), functional recombinant expression of mutant I44F in Rhodobacter sphaeroides photosynthesis-deficient mutant strain BG1 that lacks a functional bchG gene encoding for bacteriochlorophyll synthase, EC 2.5.1.133 | Synechocystis sp. PCC 6803 |
Protein Variants | Comment | Organism |
---|---|---|
I44F | naturyll occuring mutant, that also shows bacteriochlorophyl syntase activity, EC 2.5.1.133 | Synechocystis sp. PCC 6803 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
chlorophyllide a | pH 7.6, 30°C, recombinant wild-type enzyme | Synechocystis sp. PCC 6803 | |
0.11 | - |
chlorophyllide a | pH 7.6, 30°C, recombinant enzyme mutant I44F | Synechocystis sp. PCC 6803 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + phytyl diphosphate | Synechocystis sp. PCC 6803 | - |
chlorophyll a + diphosphate | - |
? | |
additional information | Synechocystis sp. PCC 6803 | chlorophyll synthase has a high degree of substrate specificity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | Q55145 | gene name chlG | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + phytyl diphosphate | - |
Synechocystis sp. PCC 6803 | chlorophyll a + diphosphate | - |
? | |
additional information | chlorophyll synthase has a high degree of substrate specificity | Synechocystis sp. PCC 6803 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChlG | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Synechocystis sp. PCC 6803 |
General Information | Comment | Organism |
---|---|---|
evolution | the predicted sequence of ChlG of Synechocystis sp. PCC 6803 is considerably similar (35% identity) to that of Rhodobacter sphaeroides BchG, each enzyme has a high level of substrate specificity to distinguish its own substrate from the other. But the BchG activity of Synechocystis chlorophyll synthase mutant ChlGI44F and the ChlG activity of bacteriochlorophyll synthase mutant BchGF28I suggest that ChlG/EC 2.5.1.62 and BchG/EC 2.5.1.133 are evolutionarily related enzymes | Synechocystis sp. PCC 6803 |
additional information | residue I44 is important for the substrate specificities of ChlG | Synechocystis sp. PCC 6803 |