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Literature summary for 2.5.1.62 extracted from

  • Kim, E.J.; Kim, H.; Lee, J.K.
    The photoheterotrophic growth of bacteriochlorophyll synthase-deficient mutant of Rhodobacter sphaeroides is restored by I44F mutant chlorophyll synthase of Synechocystis sp. PCC 6803 (2016), J. Microbiol. Biotechnol., 26, 959-966.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene chlG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), functional recombinant expression of mutant I44F in Rhodobacter sphaeroides photosynthesis-deficient mutant strain BG1 that lacks a functional bchG gene encoding for bacteriochlorophyll synthase, EC 2.5.1.133 Synechocystis sp. PCC 6803

Protein Variants

Protein Variants Comment Organism
I44F naturyll occuring mutant, that also shows bacteriochlorophyl syntase activity, EC 2.5.1.133 Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
chlorophyllide a pH 7.6, 30°C, recombinant wild-type enzyme Synechocystis sp. PCC 6803
0.11
-
chlorophyllide a pH 7.6, 30°C, recombinant enzyme mutant I44F Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyllide a + phytyl diphosphate Synechocystis sp. PCC 6803
-
chlorophyll a + diphosphate
-
?
additional information Synechocystis sp. PCC 6803 chlorophyll synthase has a high degree of substrate specificity ?
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 Q55145 gene name chlG
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + phytyl diphosphate
-
Synechocystis sp. PCC 6803 chlorophyll a + diphosphate
-
?
additional information chlorophyll synthase has a high degree of substrate specificity Synechocystis sp. PCC 6803 ?
-
?

Synonyms

Synonyms Comment Organism
ChlG
-
Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Synechocystis sp. PCC 6803

General Information

General Information Comment Organism
evolution the predicted sequence of ChlG of Synechocystis sp. PCC 6803 is considerably similar (35% identity) to that of Rhodobacter sphaeroides BchG, each enzyme has a high level of substrate specificity to distinguish its own substrate from the other. But the BchG activity of Synechocystis chlorophyll synthase mutant ChlGI44F and the ChlG activity of bacteriochlorophyll synthase mutant BchGF28I suggest that ChlG/EC 2.5.1.62 and BchG/EC 2.5.1.133 are evolutionarily related enzymes Synechocystis sp. PCC 6803
additional information residue I44 is important for the substrate specificities of ChlG Synechocystis sp. PCC 6803