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Literature summary for 2.5.1.62 extracted from

  • Hitchcock, A.; Jackson, P.J.; Chidgey, J.W.; Dickman, M.J.; Hunter, C.N.; Canniffe, D.P.
    Biosynthesis of chlorophyll a in a purple bacterial phototroph and assembly into a plant chlorophyll-protein complex (2016), ACS Synth. Biol., 5, 948-954.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene chlG, functional recombinant expression of codon-optimizes Synechocystis sp. gene chlG with Synechocystis sp. gene chlP in Rhodobacter sphaeroides lacking genes bchlG and bchlP, mutant subcloning in Escherichia coli strains JM109 and S17-1 Synechocystis sp. PCC 6803

Protein Variants

Protein Variants Comment Organism
additional information improvements to photosynthetic efficiency could be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption via development of organisms that can produce both bacteriochlorophylls and chlorophylls, engineering of the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase results in the production of chlorophyll a. Chlorophyll a can be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, method optimization, overview Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 Q55145 gene chlG
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + phytyl diphosphate engineered enzyme mutant Synechocystis sp. PCC 6803 chlorophyll a + diphosphate
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Synonyms

Synonyms Comment Organism
ChlG
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Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Synechocystis sp. PCC 6803