C109A |
mutant enzyme exhibits nearly no enzymatic activity, N-phenylmaleimide results in an additional decrease of activity |
Avena sativa |
C130A |
mutant enzyme shows reduced activity and sensitivity to N-phenylmaleimide |
Avena sativa |
C137A |
mutant enzyme is not impaired in enzymatic activity and shows the same inhibition by N-phenylmaleimide as the wild-type enzyme |
Avena sativa |
C262A |
mutant enzyme is not impaired in enzymatic activity and shows the same inhibition by N-phenylmaleimide as the wild-type enzyme |
Avena sativa |
C304A |
as active as the wild-type enzyme, mutant enzyme is not inhibited by N-phenylmaleimide |
Avena sativa |
additional information |
deletion of the presequence yields a protein with full activity, even further deletion of the N-terminus including amino acid residues 1-87 results in a core protein that is still enzymatically active. Deletion of the 88 N-terminal residues yields a protein without enzymatic activity. At the C-terminus, only one residue H378 can be deleted without loss of activity, while deletion of S77 together with H378, and all shorter sequences show no activity |
Avena sativa |
R151A |
mutant enzyme with 35% of the activity compared to wild-type enzyme |
Avena sativa |
R161A |
mutant enzyme without activity |
Avena sativa |
R284A |
mutant enzyme shows wild-type activity |
Avena sativa |
R91A |
mutant enzyme without activity |
Avena sativa |