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Literature summary for 2.5.1.61 extracted from
- Purification by affinity chromatography and properties of uroporphyrinogen I synthetase from Chlorella regularis (1980), Biochim. Biophys. Acta, 616, 300-309.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | - |
Cereibacter sphaeroides |  |
| N-ethylmaleimide | 1 mM, 59% inhibition | Chlorella regularis | |
| N-ethylmaleimide | - |
Triticum aestivum | |
| p-chloromercuribenzoate | - |
Cereibacter sphaeroides | |
| p-chloromercuribenzoate | 0.025 mM, 62% inhibition | Chlorella regularis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
porphobilinogen | - |
Triticum aestivum | |
| 0.072 | - |
porphobilinogen | - |
Spinacia oleracea | |
| 0.085 | - |
Hydroxymethylbilane | - |
Chlorella regularis | |
| 0.089 | - |
porphobilinogen | - |
Chlorella regularis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, calculation from amino acid residues | Chlorella regularis |
| 35000 | 36000 | gel filtration | Chlorella regularis |
| 38000 | - |
sucrose density gradient centrifugation | Spinacia oleracea |
| 40000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| porphobilinogen | Triticum aestivum | - |
? | - |
? | |
| porphobilinogen | Cereibacter sphaeroides | - |
? | - |
? | |
| porphobilinogen | Spinacia oleracea | - |
? | - |
? | |
| porphobilinogen | Chlorella regularis | - |
? | - |
? | |
| porphobilinogen | Chlorella regularis S-50 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
| Chlorella regularis | - |
S-50 | - |
| Chlorella regularis S-50 | - |
S-50 | - |
| Spinacia oleracea | - |
spinach | - |
| Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlorella regularis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0058 | - |
- |
Chlorella regularis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Triticum aestivum | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Cereibacter sphaeroides | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Spinacia oleracea | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Chlorella regularis | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Chlorella regularis S-50 | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Triticum aestivum | hydroxylmethylbilane + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Spinacia oleracea | hydroxylmethylbilane + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Cereibacter sphaeroides | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
| 4 porphobilinogen + H2O | - |
Chlorella regularis | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
| 4 porphobilinogen + H2O | - |
Chlorella regularis S-50 | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
| porphobilinogen | - |
Triticum aestivum | ? | - |
? | |
| porphobilinogen | - |
Cereibacter sphaeroides | ? | - |
? | |
| porphobilinogen | - |
Spinacia oleracea | ? | - |
? | |
| porphobilinogen | - |
Chlorella regularis | ? | - |
? | |
| porphobilinogen | - |
Chlorella regularis S-50 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Cereibacter sphaeroides |
| monomer | 1 * 33000, calculation from amino acid residues | Chlorella regularis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| uroporphyrinogen I synthetase | - |
Cereibacter sphaeroides |
| uroporphyrinogen I synthetase | - |
Spinacia oleracea |
| uroporphyrinogen I synthetase | - |
Chlorella regularis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
24% above the activity at 37°C | Chlorella regularis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | 10% of maximal activity at 20°C, 8% of maximal activity at 80°C | Chlorella regularis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
1 h, little loss of activity | Chlorella regularis |
| 75 | - |
13% loss of activity after 1h | Chlorella regularis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
both in phosphate buffer and Tris buffer | Chlorella regularis |
| 7.8 | 8.2 | - |
Cereibacter sphaeroides |
| 8.2 | - |
- |
Triticum aestivum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Chlorella regularis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | stable between | Chlorella regularis |
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