Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Cereibacter sphaeroides | |
N-ethylmaleimide | 1 mM, 59% inhibition | Chlorella regularis | |
N-ethylmaleimide | - |
Triticum aestivum | |
p-chloromercuribenzoate | - |
Cereibacter sphaeroides | |
p-chloromercuribenzoate | 0.025 mM, 62% inhibition | Chlorella regularis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
porphobilinogen | - |
Triticum aestivum | |
0.072 | - |
porphobilinogen | - |
Spinacia oleracea | |
0.085 | - |
Hydroxymethylbilane | - |
Chlorella regularis | |
0.089 | - |
porphobilinogen | - |
Chlorella regularis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 33000, calculation from amino acid residues | Chlorella regularis |
35000 | 36000 | gel filtration | Chlorella regularis |
38000 | - |
sucrose density gradient centrifugation | Spinacia oleracea |
40000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
porphobilinogen | Triticum aestivum | - |
? | - |
? | |
porphobilinogen | Cereibacter sphaeroides | - |
? | - |
? | |
porphobilinogen | Spinacia oleracea | - |
? | - |
? | |
porphobilinogen | Chlorella regularis | - |
? | - |
? | |
porphobilinogen | Chlorella regularis S-50 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Chlorella regularis | - |
S-50 | - |
Chlorella regularis S-50 | - |
S-50 | - |
Spinacia oleracea | - |
spinach | - |
Triticum aestivum | - |
wheat | - |
Purification (Comment) | Organism |
---|---|
- |
Chlorella regularis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
germ | - |
Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0058 | - |
- |
Chlorella regularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Triticum aestivum | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Cereibacter sphaeroides | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Spinacia oleracea | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Chlorella regularis | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Chlorella regularis S-50 | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Triticum aestivum | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Spinacia oleracea | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Cereibacter sphaeroides | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
4 porphobilinogen + H2O | - |
Chlorella regularis | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
4 porphobilinogen + H2O | - |
Chlorella regularis S-50 | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
porphobilinogen | - |
Triticum aestivum | ? | - |
? | |
porphobilinogen | - |
Cereibacter sphaeroides | ? | - |
? | |
porphobilinogen | - |
Spinacia oleracea | ? | - |
? | |
porphobilinogen | - |
Chlorella regularis | ? | - |
? | |
porphobilinogen | - |
Chlorella regularis S-50 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Cereibacter sphaeroides |
monomer | 1 * 33000, calculation from amino acid residues | Chlorella regularis |
Synonyms | Comment | Organism |
---|---|---|
uroporphyrinogen I synthetase | - |
Cereibacter sphaeroides |
uroporphyrinogen I synthetase | - |
Spinacia oleracea |
uroporphyrinogen I synthetase | - |
Chlorella regularis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
24% above the activity at 37°C | Chlorella regularis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | 10% of maximal activity at 20°C, 8% of maximal activity at 80°C | Chlorella regularis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
1 h, little loss of activity | Chlorella regularis |
75 | - |
13% loss of activity after 1h | Chlorella regularis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
both in phosphate buffer and Tris buffer | Chlorella regularis |
7.8 | 8.2 | - |
Cereibacter sphaeroides |
8.2 | - |
- |
Triticum aestivum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Chlorella regularis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | stable between | Chlorella regularis |