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Literature summary for 2.5.1.60 extracted from
- Arabidopsis Rab geranylgeranyltransferases demonstrate redundancy and broad substrate specificity in vitro (2016), J. Biol. Chem., 291, 1398-1410.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RGTA1, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of c-Myc-tagged RGTA1 subunit in Saccharomyces cerevisiae strain YPH499 | Arabidopsis thaliana |
| gene RGTA2, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of c-Myc-tagged RGTA2 subunit in Saccharomyces cerevisiae strain YPH499. No detectable RGTA2-c-Myc is present in the pulldown eluate, indicating that RGTA2-c-Myc can only be pulled down in a complex with FLAG-tagged RGTB1/2 | Arabidopsis thaliana |
| gene RGTB1, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of FLAG-tagged RGTB1 subunit in Saccharomyces cerevisiae strain YPH499 | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonia | - |
Arabidopsis thaliana |  |
| Isopropyl alcohol | - |
Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q84J75 | gene rgtb1 | - |
| Arabidopsis thaliana | Q8VYB7 | gene rgta1 encoding the alpha 1 subunit of Rab-GGT | - |
| Arabidopsis thaliana | Q9FJ32 | gene rgta2 | - |
| Arabidopsis thaliana | Q9LHL5 | gene rgtb2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged and c-Myc-tagged RGTA1 subunit from Saccharomyces cerevisiae strain YPH499 by affinity chromatography | Arabidopsis thaliana |
| recombinant His-tagged and FLAG-tagged RGTB1 subunit from Saccharomyces cerevisiae strain YPH499 by affinity chromatography | Arabidopsis thaliana |
| recombinant His-tagged and FLAG-tagged RGTB2 subunit from Saccharomyces cerevisiae strain YPH499 by affinity chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana Rab geranylgeranyltransferases demonstrate redundancy and broad substrate specificity in vitro, comparisons of isozymes and subunits, respectively, overview. RGTB1 and RGTB2 are biochemically redundant Rab-GGT alpha-subunits in Arabidopsis, whereas RGTA1 is the active alpha subunit, while RGTA2 is not. Rab-GGT alpha-subunits in Arabidopsis partner with putative beta-subunits as functional Rab-GGTs. The two subunits in all of the four combinations physically interact with each other, interaction analysis | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtRGTA1 | UniProt | Arabidopsis thaliana |
| AtRGTA2 | UniProt | Arabidopsis thaliana |
| AtRGTB1 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type I subunit alpha 1 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type I subunit alpha 2 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type II subunit beta 1 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type II subunit beta 2 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type-1 subunit alpha 1 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type-2 subunit alpha 2 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type-2 subunit beta 1 | UniProt | Arabidopsis thaliana |
| geranylgeranyl transferase type-2 subunit beta 2 | UniProt | Arabidopsis thaliana |
| Rab geranylgeranyl transferase alpha subunit 2 | UniProt | Arabidopsis thaliana |
| Rab geranylgeranyl transferase beta subunit 1 | UniProt | Arabidopsis thaliana |
| Rab geranylgeranyl transferase beta subunit 2 | UniProt | Arabidopsis thaliana |
| Rab geranylgeranyl transferase lpha subunit 1 | UniProt | Arabidopsis thaliana |
| Rab geranylgeranyltransferase | - |
Arabidopsis thaliana |
| RAB-GGT | - |
Arabidopsis thaliana |
| Rab-GGT alpha 1 | UniProt | Arabidopsis thaliana |
| Rab-GGT alpha 2 | UniProt | Arabidopsis thaliana |
| Rab-GGT beta 1 | UniProt | Arabidopsis thaliana |
| Rab-GGT beta 2 | UniProt | Arabidopsis thaliana |
| RGTA1 | - |
Arabidopsis thaliana |
| RGTA2 | - |
Arabidopsis thaliana |
| RGTB1 | - |
Arabidopsis thaliana |
| RGTB2 | - |
Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Arabidopsis RGTB1 and RGTB2 are biochemically redundant in vitro, suggesting that there might be additional factors that differentiate RGTB1 and RGTB2 functions in vivo. Alternatively, the partial redundancy of RGTB1 and RGTB2 in Arabidopsis may result from differential expression | Arabidopsis thaliana |
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