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Literature summary for 2.5.1.60 extracted from

  • Hala, M.; Soukupova, H.; Synek, L.; Zarsky, V.
    Arabidopsis RAB geranylgeranyl transferase beta-subunit mutant is constitutively photomorphogenic, and has shoot growth and gravitropic defects (2010), Plant J., 62, 615-627.
    View publication on PubMed
Search for more literature for 2.5.1.60

Protein Variants

Protein Variants Comment Organism
additional information homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%) and result in unprenylated RAB GTPase accumulation in the cytoplasm, up-regulation (50% and more) of about one third of RAB-GTPase paralogs in dark-grown plants compared to light-grown plants, down-regulation of about one tenth, endocytosis and exocytosis are downregulated in homozygotes defective in shoot growth and morphogenesis, root gravitropism is normal but compromised in shoots, etiolation is defective, mutants show constitutive photomorphogenic phenotypes, smaller rosette diameter, loss of apical dominance Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + Arabidopsis thaliana RAB-ATa protein Arabidopsis thaliana homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%), 50 mM phosphate buffer, pH 7.6, 10 mM MgCl2, 5 mM DTT, 0.5 microM tritium-labelled all trans-geranylgeranyl pyrophosphate, 4 microg recombinant Arabidopsis thaliana RAB-A2a protein, 32°C, 40 min S-geranylgeranyl-RAB GTPase + diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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Purification (Commentary)

Purification (Comment) Organism
fresh plant material is frozen in liquid nitrogen, ground on ice with acid-washed sand in 100 mM Tris buffer, pH 7.8, containing 5 mM EGTA, 5 mM EDTA, 10 mM beta-mercaptoethanol, and 10% glycerol, lysate is centrifuged, dialyzed against 50 mM Tris buffer, pH 7.8, containing 10% glycerol, and 1 mM DTT Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
gametophyte both beta subunit levels are similar in male gametophytes, subunit A1 is expressed in all tissues, subunit A2 is expressed significantly only in pollen Arabidopsis thaliana
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sporophyte subunit B1 is about 10times more abundant in sporophytes than subunit B2, subunit A1 is expressed in all tissues Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + Arabidopsis thaliana RAB-ATa protein homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%), 50 mM phosphate buffer, pH 7.6, 10 mM MgCl2, 5 mM DTT, 0.5 microM tritium-labelled all trans-geranylgeranyl pyrophosphate, 4 microg recombinant Arabidopsis thaliana RAB-A2a protein, 32°C, 40 min Arabidopsis thaliana S-geranylgeranyl-RAB GTPase + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
Rab geranylgeranyltransferase type II
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 Arabidopsis thaliana
RAB-GGT
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 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction homozygous mutants of the major beta-subunit of Arabidopsis enzyme (RGTB1) result in unprenylated RAB GTPase accumulation in the cytoplasm, up-regulation of several GTPases, endocytosis and exocytosis are downregulated, defects in shoot growth and morphogenesis, changed shoot gravitropism and reaction to etiolation Arabidopsis thaliana
physiological function membrane localization of RAB GTPase is mediated by C-terminal double geranylgeranylation by the enzyme in cooperation with the RAB escort protein (REP) Arabidopsis thaliana