Literature summary for 2.5.1.60 extracted from

Wu, Y.W.; Goody, R.S.; Abagyan, R.; Alexandrov, K.
Structure of the disordered C terminus of Rab7 GTPase induced by binding to the Rab geranylgeranyl transferase catalytic complex reveals the mechanism of Rab prenylation (2009), J. Biol. Chem., 284, 13185-13192.

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Protein Variants

Protein Variants	Comment	Organism
additional information	mutant Rab7 substrates with truncated C-terminal show that a lack of more than 3 residues leads to dramatic reduction in Rab prenylation efficiency of the enzyme, residual prenylation activity with 4-5 lacking residues, further reduction abolishes the ability of Rab7 to be an enzyme substrate	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E,10Z)-3,7,11-trimethyl-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodeca-2,6,10-trien-1-yl diphosphate + Rab7 GTPase	in vitro prenylation assay with 4 microM Rab7 wild type or mutants, 6 mircoM Rab escort protein, 6 microM enzyme, 50 mM HEPES buffer, pH 7.2, containing 50 mM NaCl, 5 mM dithioerythritol, 2 mM MgCl2, and 10 mircoM GDP, 25°C, 50 microM of the geranylgeranyl diphosphate analogue (3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,10-triem-1) diphosphate, end-point assay with 6 microM Rab7 wild type or mutants, 10 microM Rab escort protein, 6 microM enzyme in the same buffer mixture, and 40 microM (3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,10-triem-1) diphosphate, 1.5 hours at 25°C	Homo sapiens	(2E,6E,10Z)-3,7,11-trimethyl-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodeca-2,6,10-trien-1-yl-Rab7 GTPase + diphosphate	-	?

Subunits

Subunits	Comment	Organism
heterodimer	2 * ?, computational model based on crystals of subcomplexes	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Rab geranylgeranyltransferase	-	Homo sapiens
RabGGTase	-	Homo sapiens

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Release 2023.1 (January 2023)