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Literature summary for 2.5.1.6 extracted from

  • Delgado, M.; Perez-Miguelsanz, J.; Garrido, F.; Rodriguez-Tarduchy, G.; Perez-Sala, D.; Pajares, M.A.
    Early effects of copper accumulation on methionine metabolism (2008), Cell. Mol. Life Sci., 65, 2080-2090.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-methionine 17% increase is observed at 60 mM L-methionine Rattus norvegicus
L-methionine 17% increase of activity is observed at 60 mM L-methionine Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ isozyme subunit MATalpha2 is inhibited by 0.25 mM Cu2+ in the presence or absence of dithiothreitol, strong reduction in MAT2B gene expression induced by Cu2+ (60%), copper effects can only be prevented by buthionine sulfoximine, whereas N-acetylcysteine and neocuproine are ineffective Rattus norvegicus
L-methionine 30% reduction in total activity is detected at 5 mM L-methionine; 30% reduction in total activity is detected at 5 mM L-methionine; 30% reduction in total activity is detected at 5 mM L-methionine Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
isozyme MAT III, gel filtration Rattus norvegicus
200000
-
isozyme MAT I, gel filtration Rattus norvegicus
200000
-
isozyme MAT II, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus P13444
-
-
Rattus norvegicus P18298
-
-

Renatured (Commentary)

Renatured (Comment) Organism
dithiothreitol-refolding in the presence or absence of 250 mM CuSO4 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Rattus norvegicus phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Subunits

Subunits Comment Organism
dimer isozyme MAT III Rattus norvegicus
tetramer isozyme MAT I Rattus norvegicus
tetramer isozyme MAT II Rattus norvegicus

Synonyms

Synonyms Comment Organism
MAT I isozyme Rattus norvegicus
MAT II isozyme Rattus norvegicus
MAT III isozyme Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.287
-
isozyme subunit MATalpha2, in the absence of dithiothreitol Rattus norvegicus Cu2+
0.748
-
isozyme subunit MATalpha2, in the presence of dithiothreitol Rattus norvegicus Cu2+