Literature summary for 2.5.1.6 extracted from

Taylor, J.C.; Takusagawa, F.; Markham, G.D.
The active site loop of S-adenosylmethionine synthetase modulates catalytic efficiency (2002), Biochemistry, 41, 9358-9369.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0013	-	tripolyphosphate	wild type, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine	Escherichia coli
0.0015	-	tripolyphosphate	G7 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine	Escherichia coli
0.0016	-	tripolyphosphate	G8 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine	Escherichia coli
0.003	-	tripolyphosphate	wild type, tripolyphosphatase activity, in absence of S-adenosylmethionine	Escherichia coli
0.0032	-	tripolyphosphate	G6 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine	Escherichia coli
0.0053	-	tripolyphosphate	G5 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine	Escherichia coli
0.008	-	tripolyphosphate	G8 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine	Escherichia coli
0.011	-	tripolyphosphate	G6 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine	Escherichia coli
0.014	-	tripolyphosphate	RLL mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine	Escherichia coli
0.015	-	tripolyphosphate	G7 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine	Escherichia coli
0.024	-	tripolyphosphate	G5 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine	Escherichia coli
0.026	-	tripolyphosphate	RLL mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine	Escherichia coli
0.026	-	ATP	G6 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.045	-	ATP	RLL and G8 mutants, S-adenosylmethionine synthesis	Escherichia coli
0.069	-	ATP	G7 mutants, S-adenosylmethionine synthesis	Escherichia coli
0.073	-	ATP	wild-type S-adenosylmethionine synthesis	Escherichia coli
0.087	-	ATP	G5 mutant S-adenosylmethionine synthesis	Escherichia coli
0.092	-	L-methionine	wild-type, S-adenosylmethionine synthesis	Escherichia coli
0.23	-	L-methionine	RLL mutant, S-adenosylmethionine synthesis	Escherichia coli
0.3	-	L-methionine	G6 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.49	-	L-methionine	G7 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.62	-	L-methionine	G8 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.77	-	L-methionine	G5 mutant, S-adenosylmethionine synthesis	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A817	XL1-Blue strain	-
Escherichia coli XL1-Blue	P0A817	XL1-Blue strain	-

Purification (Commentary)

Purification (Comment)	Organism
method that includes ammonium sulfate fraction, phenyl-Sepharose HR and hydroxylapetite CHT-1 chromatographies and amminohexyl-Sepharose anion exchange	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + L-methionine + H2O	-	Escherichia coli	S-adenosyl-L-methionine + phosphate + diphosphate	-	?
ATP + L-methionine + H2O	-	Escherichia coli XL1-Blue	S-adenosyl-L-methionine + phosphate + diphosphate	-	?
tripolyphosphate + H2O	-	Escherichia coli	diphosphate + phosphate	-	?
tripolyphosphate + H2O	-	Escherichia coli XL1-Blue	diphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	gel filtration and native gel electrophoresis	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.000000217	-	tripolyphosphate	G8 mutant, tripolyphosphatase activity	Escherichia coli
0.000000367	-	S-adenosylmethionine	RLL mutant, S-adenosylmethionine synthesis	Escherichia coli
0.00000045	-	S-adenosylmethionine	G6 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.000000617	-	tripolyphosphate	G6 mutant, tripolyphosphatase activity	Escherichia coli
0.000000633	-	tripolyphosphate	G5 mutant, tripolyphosphatase activity	Escherichia coli
0.000000667	-	S-adenosylmethionine	G8 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.00000122	-	tripolyphosphate	RLL and G7 mutants, tripolyphosphatase activity	Escherichia coli
0.00000167	-	tripolyphosphate	wild-type, tripolyphosphatase activity	Escherichia coli
0.00000217	-	S-adenosylmethionine	G5 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.000012	-	S-adenosylmethionine	G7 mutant, S-adenosylmethionine synthesis	Escherichia coli
0.000417	-	S-adenosylmethionine	wild-type, S-adenosylmethionine synthesis	Escherichia coli