Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for reducing agents | Saccharolobus solfataricus | |
S-adenosylmethionine | activates the tripolyphosphatase activity of A isoform | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
ATP, preincubation protects against thermal inactivation | Saccharolobus solfataricus |
glycerol, 10% stabilizes | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | 20 mM, 37% inhibition, S-adenosylmethionine synthetase B; 20 mM, 40% inhibition, S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
cycloleucine | 25 mM, 56% inhibition, S-adenosylmethionine synthetase A; inhibits only at sub saturating concentrations of methionine | Saccharolobus solfataricus | |
diphosphate | 20 mM, 30% inhibition, S-adenosylmethionine synthetase A; 20 mM, 49% inhibition, S-adenosylmethionine synthetase B | Saccharolobus solfataricus | |
GTP | 20 mM, 50% inhibition, S-adenosylmethionine synthetase B; 20 mM, 56% inhibition, S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
additional information | S-adenosyl(5')-3-methylthiopropylamine does not inhibit | Saccharolobus solfataricus | |
phosphate | 10 mM, 19% inhibition, S-adenosylmethionine synthetase B; 10 mM, 45% inhibition, S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
S-adenosyl-L-homocysteine | not inhibitory | Saccharolobus solfataricus | |
S-adenosylmethionine | inhibits the A form but not the B form; non-competitive, S-adenosylmethionine synthetase A; slight inhibition of S-adenosylmethionine synthetase B | Saccharolobus solfataricus | |
Tetrapolyphosphate | 10 mM, 40% inhibition, S-adenosylmethionine synthetase B; 10 mM, 50% inhibition, S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
tripolyphosphate | 1.0 mM, 57% inhibition, S-adenosylmethionine synthetase A; 1.0 mM, 62% inhibition, S-adenosylmethionine synthetase B | Saccharolobus solfataricus | |
UTP | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-methionine | isoenzyme A | Saccharolobus solfataricus | |
0.01 | - |
L-methionine | S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
0.02 | - |
L-methionine | isoenzyme B | Saccharolobus solfataricus | |
0.024 | - |
L-methionine | S-adenosylmethionine synthetase B | Saccharolobus solfataricus | |
0.05 | - |
ATP | isoenzyme A | Saccharolobus solfataricus | |
0.065 | - |
ATP | S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
0.15 | - |
ATP | isoenzyme B | Saccharolobus solfataricus | |
0.156 | - |
ATP | S-adenosylmethionine synthetase B | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharolobus solfataricus | 5829 | - |
cytosol | S-adenosylmethionine synthetase A | Saccharolobus solfataricus | 5829 | - |
cytosol | S-adenosylmethionine synthetase B | Saccharolobus solfataricus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ with lower relative activity | Saccharolobus solfataricus | |
Co2+ | can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B | Saccharolobus solfataricus | |
Mg2+ | maximal activation at 40 mM | Saccharolobus solfataricus | |
Mg2+ | S-adenosylmethionine synthetase A is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM) | Saccharolobus solfataricus | |
Mg2+ | S-adenosylmethionine synthetase B is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM) | Saccharolobus solfataricus | |
Mn2+ | can replace Mg2+ with lower relative activity | Saccharolobus solfataricus | |
Mn2+ | can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase A | Saccharolobus solfataricus | |
Mn2+ | can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
isoenzyme B, gel filtration | Saccharolobus solfataricus |
75000 | - |
S-adenosylmethionine synthetase B, gel filtration | Saccharolobus solfataricus |
180000 | - |
isoenzyme A, gel filtration | Saccharolobus solfataricus |
180000 | - |
S-adenosylmethionine synthetase A, gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Saccharolobus solfataricus | - |
S-adenosyl-L-methionine + phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | - |
2 forms: A and B | - |
Purification (Comment) | Organism |
---|---|
partial, S-adenosylmethionine synthetase B | Saccharolobus solfataricus |
two isoforms: A and B | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharolobus solfataricus |
0.065 | - |
S-adenosylmethionine synthetase B | Saccharolobus solfataricus |
0.138 | - |
S-adenosylmethionine synthetase A | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, partially purified isoenzymes, 10 mM Tris/HCl, pH 8, stable for 4 weeks | Saccharolobus solfataricus |
-20°C, pH 8, 10 mM Tris/HCl buffer, partially purified S-adenosylmethionine synthetase A is stable for four weeks | Saccharolobus solfataricus |
-20°C, pH 8, 10 mM Tris/HCl buffer, partially purified S-adenosylmethionine synthetase B is stable for four weeks | Saccharolobus solfataricus |
4°C, isoenzyme A and B are spontaneously inactivated in 1 month and 1 week, respectively, in presence of glycerol stability increases up to 90% | Saccharolobus solfataricus |
4°C, S-adenosylmethionine synthetase is spontaneously inactivated in 1 month, S-adenosylmethionine synthetase A | Saccharolobus solfataricus |
4°C, S-adenosylmethionine synthetase is spontaneously inactivated in 1 week | Saccharolobus solfataricus |
are stable in buffer A at | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-ATP + L-methionine + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3'-deoxy-ATP + L-methionine + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
ATP + L-methionine + H2O | - |
Saccharolobus solfataricus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | - |
Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
additional information | S-adenosylmethionine synthetase A exhibits tripolyphosphatase activity | Saccharolobus solfataricus | ? | - |
? | |
additional information | S-adenosylmethionine synthetase B exhibits tripolyphosphatase activity | Saccharolobus solfataricus | ? | - |
? | |
tripolyphosphate + H2O | tripolyphosphatase activity | Saccharolobus solfataricus | diphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S-adenosylmethionine synthetase A | - |
Saccharolobus solfataricus |
S-adenosylmethionine synthetase B | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Saccharolobus solfataricus |
90 | - |
S-adenosylmethionine synthetase A | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 110 | about 50% of activity maximum at 75°C and 110°C | Saccharolobus solfataricus |
75 | 110 | 50% of maximal activity at 75°C and at 110°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
preincubation with ATP protects against thermal inactivation | Saccharolobus solfataricus |
100 | - |
30 min, 90% loss of activity | Saccharolobus solfataricus |
100 | - |
more than 80% activity is retained in the presence of 10 mM ATP at 100°C for 30 min. GTP, ADP, AMP and methionine do not exert any protective effect, S-adenosylmethionine synthetase A | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
- |
Saccharolobus solfataricus |
8.1 | - |
S-adenosylmethionine synthetase A | Saccharolobus solfataricus |
8.1 | - |
S-adenosylmethionine synthetase B | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.3 | about 50% of activity maximum at pH 7.2 and 9.3 | Saccharolobus solfataricus |
7.2 | 9.3 | 50% of maximal activity at pH 7.2 and at pH 9.3, S-adenosylmethionine synthetase A | Saccharolobus solfataricus |
7.2 | 9.3 | 50% of maximal activity at pH 7.2 and at pH 9.3, S-adenosylmethionine synthetase B | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
S-adenosylmethionine | inhibits the A isoform | Saccharolobus solfataricus | |
0.05 | - |
S-adenosylmethionine | S-adenosylmethionine synthetase A | Saccharolobus solfataricus |