Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Adenyl-5'-ylimidodiphosphate | competitive with ATP | Escherichia coli | |
diphosphate | - |
Escherichia coli | |
phosphate | - |
Escherichia coli | |
S-adenosylmethionine | - |
Escherichia coli | |
S-carbamylcysteine | competitive with methionine | Escherichia coli | |
tripolyphosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | for maximal activation the cation concentration must be at least equal to ATP concentration | Escherichia coli | |
Mg2+ | cation concentration must be at least equal to ATP concentration for maximal activity | Escherichia coli | |
Mn2+ | for maximal activation the cation concentration must be at least equal to ATP concentration | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000 | Escherichia coli |
180000 | - |
- |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
0.75 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 5 mg/ml enzyme concentration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-ATP + L-methionine + H2O | - |
Escherichia coli | ? | - |
? | |
3'-deoxy-ATP + L-methionine + H2O | - |
Escherichia coli | ? | - |
? | |
ATP + L-methionine + H2O | - |
Escherichia coli | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
additional information | ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate | Escherichia coli | ? | - |
? | |
tripolyphosphate + H2O | tripolyphosphatase activity | Escherichia coli | diphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000 | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |