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Literature summary for 2.5.1.59 extracted from

  • Kim, H.; Koo, S.H.; Choi, H.J.; Yang, C.H.
    Characterization of yeast geranylgeranyl transferase type I expressed in Escherichia coli (1996), Mol. Cell, 6, 602-608.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
alpha-subunit encoded by RAM2 cloned to the pFC vector, which has an chloroamphenicol resistance gene and beta-subunit encoded by CAL1 cloned to the pFlag vector, which has an ampicillin resistance gene, these two recombinant enzymes are cotransformed and expressed in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
geranylgeranyl diphosphate pH 7.5, 37°C Saccharomyces cerevisiae
0.00143
-
[glutathione S-transferase]-GCVKIKKCVIL pH 7.5, 37°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal activity of EDTA-treated enzyme at 0.5 mM, Mg2+ probably activates the diphosphate leaving group of enzyme, and is essential for transferring the bound peptide substrate Saccharomyces cerevisiae
Zn2+ enzyme contains tightly bound Zn2+ which is required for its catalytic activity Bos taurus
Zn2+ requirement, optimal activity of EDTA-treated enzyme at 0.01 mM in presence of 1 mM Mg2+ Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + protein-cysteine Saccharomyces cerevisiae
-
S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine Bos taurus
-
S-geranylgeranyl-protein + diphosphate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information no enzyme activity in the presence of EDTA Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + protein-cysteine
-
Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine
-
Bos taurus S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Bos taurus S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Bos taurus S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL
-
Saccharomyces cerevisiae diphosphate + ?
-
?
geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL
-
Bos taurus diphosphate + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.3
-
Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.6
-
at pH 8.6 no enzyme activity is detected Saccharomyces cerevisiae