Cloned (Comment) | Organism |
---|---|
alpha-subunit encoded by RAM2 cloned to the pFC vector, which has an chloroamphenicol resistance gene and beta-subunit encoded by CAL1 cloned to the pFlag vector, which has an ampicillin resistance gene, these two recombinant enzymes are cotransformed and expressed in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C | Saccharomyces cerevisiae | |
0.00143 | - |
[glutathione S-transferase]-GCVKIKKCVIL | pH 7.5, 37°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal activity of EDTA-treated enzyme at 0.5 mM, Mg2+ probably activates the diphosphate leaving group of enzyme, and is essential for transferring the bound peptide substrate | Saccharomyces cerevisiae | |
Zn2+ | enzyme contains tightly bound Zn2+ which is required for its catalytic activity | Bos taurus | |
Zn2+ | requirement, optimal activity of EDTA-treated enzyme at 0.01 mM in presence of 1 mM Mg2+ | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine | Saccharomyces cerevisiae | - |
S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | Bos taurus | - |
S-geranylgeranyl-protein + diphosphate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | no enzyme activity in the presence of EDTA | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine | - |
Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | - |
Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu | Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X | Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL | - |
Saccharomyces cerevisiae | diphosphate + ? | - |
? | |
geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL | - |
Bos taurus | diphosphate + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.3 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
at pH 8.6 no enzyme activity is detected | Saccharomyces cerevisiae |