Cloned (Comment) | Organism |
---|---|
alpha-subunit encoded by RAM2 and beta-subunit encoded by CDC43 translationally coupled by overlapping the RAM-CDC43 stop-start codons and by locating a ribosome-binding site near the 3' end of RAM2, recombinant enzyme overproduced in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Saccharomyces cerevisiae | |
0.0024 | - |
dansyl-Gly-Cys-Ile-Ile-Leu | pH 7.5, 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement, optimal activity at 0.5 mM in presence of 0.1 mM Zn2+ | Saccharomyces cerevisiae | |
Zn2+ | requirement, optimal activity of EDTA-treated enzyme at 0.01 mM in presence of 1 mM Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
alpha,beta, 1 * 34000 + 1 * 42000 | Saccharomyces cerevisiae |
42000 | - |
alpha,beta, 1 * 34000 + 1 * 42000 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine | Saccharomyces cerevisiae | - |
S-geranylgeranyl-protein + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate | this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + dansyl-Cys-Ile-Ile-Leu | - |
Saccharomyces cerevisiae | diphosphate + dansyl-S-geranylgeranyl-Cys-Ile-Ile-Leu | - |
? | |
geranylgeranyl diphosphate + dansyl-Gly-Cys-Ile-Ile-Leu | - |
Saccharomyces cerevisiae | dansyl-Gly-(S-geranylgeranyl)-Cys-Ile-Ile-Leu + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | - |
Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alpha,beta, 1 * 34000 + 1 * 42000 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Saccharomyces cerevisiae |