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Literature summary for 2.5.1.59 extracted from

  • Stirtan, W.G.; Poulter, C.D.
    Yeast protein geranylgeranyltransferase type-I: overproduction, purification, and characterization (1995), Arch. Biochem. Biophys., 321, 182-190.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
alpha-subunit encoded by RAM2 and beta-subunit encoded by CDC43 translationally coupled by overlapping the RAM-CDC43 stop-start codons and by locating a ribosome-binding site near the 3' end of RAM2, recombinant enzyme overproduced in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
geranylgeranyl diphosphate pH 7.5, 30°C Saccharomyces cerevisiae
0.0024
-
dansyl-Gly-Cys-Ile-Ile-Leu pH 7.5, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement, optimal activity at 0.5 mM in presence of 0.1 mM Zn2+ Saccharomyces cerevisiae
Zn2+ requirement, optimal activity of EDTA-treated enzyme at 0.01 mM in presence of 1 mM Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
alpha,beta, 1 * 34000 + 1 * 42000 Saccharomyces cerevisiae
42000
-
alpha,beta, 1 * 34000 + 1 * 42000 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + protein-cysteine Saccharomyces cerevisiae
-
S-geranylgeranyl-protein + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + dansyl-Cys-Ile-Ile-Leu
-
Saccharomyces cerevisiae diphosphate + dansyl-S-geranylgeranyl-Cys-Ile-Ile-Leu
-
?
geranylgeranyl diphosphate + dansyl-Gly-Cys-Ile-Ile-Leu
-
Saccharomyces cerevisiae dansyl-Gly-(S-geranylgeranyl)-Cys-Ile-Ile-Leu + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine
-
Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?

Subunits

Subunits Comment Organism
heterodimer alpha,beta, 1 * 34000 + 1 * 42000 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Saccharomyces cerevisiae