Literature summary for 2.5.1.59 extracted from

Zhang, F.L.; Kirschmeier, P.; Carr, D.; James, L.; Bond, R.W.; Wang, L.; Patton, R.; Windsor, W.T.; Syto, R.; Zhang, R.; Bishop, W.R.
Characterization of Ha-Ras, N-Ras, Ki-Ras4A, and Ki-Ras4B as in vitro substrates for farnesyl protein transferase and geranylgeranyl protein transferase type I (1997), J. Biol. Chem., 272, 10232-10239.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0009	-	Ras-Cys-Val-Leu-Leu	pH 7.5, 37°C	Homo sapiens
0.0021	-	N-Ras	pH 7.5, 37°C	Homo sapiens
0.0088	-	Ki-Ras4A	pH 7.5, 37°C	Homo sapiens
0.012	-	Ki-Ras4B	pH 7.5, 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	enzyme produced in Sf9cells using baculovirus system	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
farnesyl diphosphate + Ki-Ras4B	-	Homo sapiens	diphosphate + S-geranylgeranyl-Ki-Ras4B	-	?
geranylgeranyl diphosphate + Ki-Ras4A	-	Homo sapiens	diphosphate + S-geranylgeranyl-Ki-Ras4A	-	?
geranylgeranyl diphosphate + N-Ras	-	Homo sapiens	diphosphate + S-geranylgeranyl-N-Ras	-	?
geranylgeranyl diphosphate + protein-cysteine	enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu	Homo sapiens	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + protein-cysteine	enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X	Homo sapiens	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + Ras-Cys-Val-Leu-Leu	-	Homo sapiens	diphosphate + Ras-S-geranylgeranyl-Cys-Val-Leu-Leu	-	?
geranylgeranyl diphosphate + S-geranylgeranyl-Ki-Ras4B	both the polylysine and the carboxy-terminal methionine are important for geranylgeranylation of this substrate	Homo sapiens	diphosphate + S-geranylgeranyl-Ki-Ras4B	-	?
additional information	not: N-Ras	Homo sapiens	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0113	-	N-Ras	-	Homo sapiens
0.014	-	Ras-Cys-Val-Leu-Leu	-	Homo sapiens
0.0667	-	Ki-Ras4A	-	Homo sapiens
0.0767	-	Ki-Ras4B	-	Homo sapiens

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Release 2023.1 (January 2023)