Literature summary for 2.5.1.59 extracted from

Yokoyama, K.; McGeady, P.; Gelb, M.H.
Mammalian protein geranylgeranyltransferase-I: Substrate specificity, kinetic mechanism, metal requirements, and affinity labeling (1995), Biochemistry, 34, 1344-1354.

Search for more literature for 2.5.1.59

Inhibitors

Inhibitors	Comment	Organism	Structure
1-phosphono-(E,E,E)-geranylgeraniol	competitive to geranylgeranyl diphosphate, noncompetitive to GST-CDC42	Bos taurus
NPFREKKFFCAIL	biotin-gamma6, substrate inhibition at high peptide concentration	Bos taurus
PD-083176	noncompetitive to geranylgeranyl diphosphate, competitive to GST-CDC42, modest inhibitor	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	comparison of Km of CAAX-substrates with X is Leu, Phe or Ser	Bos taurus
0.00027	-	(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	pH 7.0, 30°C, prenyl donor: geranylgeranyl diphosphate	Bos taurus
0.0078	-	(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	pH 7.0, 30°C, prenyl donor: farnesyl diphosphate	Bos taurus
0.016	-	GST-CDC42	pH 7.0, 30°C, prenyl donor: geranylgeranyl diphosphate	Bos taurus
0.025	-	(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	pH 7.0, 30°C, prenyl donor: geranylgeranyl diphosphate	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	the zinc in enzyme can be replaced by Cd2+	Bos taurus
Cd2+	80% of maximal activity in the presence of 0.5 mM alone	Bos taurus
Mg2+	20% of maximal activity in the presence of 0.5 mM alone	Bos taurus
Mn2+	60% of maximal activity in the presence of 0.5 mM alone	Bos taurus
additional information	not: Co2+	Bos taurus
additional information	metals not needed for complex formation between enzyme and geranylgeranyl diphosphate, but addition of prenyl acceptor only results in prenyl transfer in presence of Mg2+ and Zn2+	Bos taurus
Zn2+	required, but no activity in presence of Zn2+ alone, only when either 0.1 mM Mg2+, Mn2+ or Cd2+ is additionally present	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate + protein-cysteine	Bos taurus	enzyme catalyses the transfer of the 20-carbon prenyl group from geranylgeranyl diphosphate to the cysteine residue near the C-termini of a variety of eukaryotic proteins	S-geranylgeranyl-protein + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Reaction

Reaction	Comment	Organism	Reaction ID
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate	random sequential mechanism	Bos taurus	a
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate	enzyme and geranylgeranyl diphosphate produce a stable binary complex and the tightly bound geranylgeranyl diphosphate is transferred to a peptide acceptor, without the equilibration of enzyme-bound geranylgeranyl diphosphate with free geranylgeranyl diphosphate	Bos taurus	a
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate	this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes	Bos taurus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
farnesyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	biotin-gamma6	Bos taurus	diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-farnesyl-CAI-Leu	-	?
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRKCCIF	-	Bos taurus	diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRK-S-geranylgeranyl-CCIF	-	?
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	biotin-gamma6, preferred substrate	Bos taurus	diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-geranylgeranyl-CAI-Leu	-	?
geranylgeranyl diphosphate + GST-CDC42	recombinant glutathione S-transferase fusion protein of CDC42Hs with a C-terminal Cys-Cys-Ile-Phe sequence	Bos taurus	diphosphate + GST-S-geranylgeranyl-CDC42	-	?
geranylgeranyl diphosphate + protein-cysteine	enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu	Bos taurus	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + protein-cysteine	enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X may be Leu, Phe, or Ser, with high concentrations of peptide substrates this specificity is lost	Bos taurus	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + protein-cysteine	enzyme catalyses the transfer of the 20-carbon prenyl group from geranylgeranyl diphosphate to the cysteine residue near the C-termini of a variety of eukaryotic proteins	Bos taurus	S-geranylgeranyl-protein + diphosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	comparison of kcat of CAAX-substrates with X is Leu, Phe or Ser	Bos taurus
0.0057	-	GST-CDC42	prenyl donor: geranylgeranyl diphosphate	Bos taurus
0.028	-	(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	prenyl donor: geranylgeranyl diphosphate	Bos taurus
0.1	-	(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	prenyl donor: farnesyl diphosphate	Bos taurus
0.12	-	(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu	prenyl donor: geranylgeranyl diphosphate	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00072	-	1-phosphono-(E,E,E)-geranylgeraniol	with respect to geranylgeranyl diphosphate	Bos taurus
0.00086	-	1-P-GGOH	with respect to GST-CDC42	Bos taurus
0.0082	-	PD-083176	with respect to GST-CDC42	Bos taurus
0.0087	-	PD-083176	with respect to geranylgeranyl diphosphate	Bos taurus

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Release 2023.1 (January 2023)