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Literature summary for 2.5.1.58 extracted from

  • Dunten, P.; Kammlott, U.; Crowther, R.; Weber, D.; Palermo, R.; Birktoft, J.
    Protein farnesyltransferase: Structure and implications for substrate binding (1998), Biochemistry, 37, 7907-7912.
    View publication on PubMed
Search for more literature for 2.5.1.58

Application

Application Comment Organism
medicine prime target for development of anticancer therapeutics Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of alpha and beta subunits of enzyme with a recombinant baculovirus Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
farnesyl diphosphate and peptide substrate can be accommodated in the hydrophobic active-site barrel, with the sole charged residue inside the barrel, Arg202 of the beta-subunit, forming a salt bridge with the negatively charged carboxy terminus of peptide substrate Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc ion is coordinated by three residues in the beta subunit: Asp-297, Cys-299, and H-362 and a water molecule Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate + protein-cysteine Rattus norvegicus process necessary for the subcellular localisation of substrate to the plasma membrane S-farnesyl protein + diphosphate
-
 ?
farnesyl diphosphate + protein-cysteine Rattus norvegicus A series of steps modifies the C-terminus of Ras. Enzyme catalyses the first of these steps by transferring the farnesyl group from farnesyl diphosphates to the cysteine side chain in the CAAX sequence motif at the C-terminus of Ras S-farnesyl protein + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate both subunits would appear to have a role in binding and orienting the leaving group of farnesyl diphosphate approximately for catalysis Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + protein-cysteine
-
 Rattus norvegicus diphosphate + S-farnesyl protein
-
 ?
farnesyl diphosphate + protein-cysteine process necessary for the subcellular localisation of substrate to the plasma membrane Rattus norvegicus S-farnesyl protein + diphosphate
-
 ?
farnesyl diphosphate + protein-cysteine A series of steps modifies the C-terminus of Ras. Enzyme catalyses the first of these steps by transferring the farnesyl group from farnesyl diphosphates to the cysteine side chain in the CAAX sequence motif at the C-terminus of Ras Rattus norvegicus S-farnesyl protein + diphosphate
-
 ?