Application | Comment | Organism |
---|---|---|
medicine | evidence that inhibitors of enzyme could be effective therapeutic agents in treatment of many human cancers | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | substitution of the active site zinc with cadmium increases the affinity of the peptide substrate and decreases the rate constant for the chemical step | Rattus norvegicus | |
Mg2+ | magnesium is not required for formation of the thioether product but the presence increases the single-turnover rate constant by several orders of magnitude at saturating enzyme and substrate concentrations | Rattus norvegicus | |
Mg2+ | appears to coordinate the diphosphate moiety of farnesyl diphosphate | Rattus norvegicus | |
additional information | a metal-assisted nucleophile is involved in the catalytic mechanism of enzyme | Rattus norvegicus | |
Zn2+ | zinc metalloenzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | Rattus norvegicus | process necessary for the subcellular localisation of substrate to the plasma membrane | S-farnesyl protein + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate | kinetic mechanism | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | prenylation, farnesylation, substrates are Ras, nuclear lamins, transducin gamma subunit, protein substrate motif: Cys-aliphatic-aliphatic-X, X: M, S, Q, A, F | Rattus norvegicus | diphosphate + S-farnesyl protein | - |
? | |
farnesyl diphosphate + protein-cysteine | process necessary for the subcellular localisation of substrate to the plasma membrane | Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of kcat of metal-substituted enzymes, kcat for product formation decreases for C3 fluoromethyl farnesyl diphosphate substrates, paralleling the number of fluorines at the C3 methyl position | Rattus norvegicus |