Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.57 extracted from

  • Watson, D.; Jourdian, G.W.; Roseman, S.
    N-Acylneuraminic (sialic) acid 9-phosphate synthetase (1966), Methods Enzymol., 8, 201-205.
No PubMed abstract available
Search for more literature for 2.5.1.57

General Stability

General Stability Organism
purified enzyme is unstable Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 N-acetyl-D-mannosamine 6-phosphate
-
 Sus scrofa
1.6
-
 N-glycolyl-D-mannosamine 6-phosphate
-
 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Sus scrofa
Mg2+ maximal enzyme activity in presence of 0.02 M Sus scrofa
Mn2+
-
 Sus scrofa
additional information Ca2+ or Cu2+ does not substitute for Mg2+ Sus scrofa
Ni2+
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Sus scrofa
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
submaxillary gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.83
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, crude extract stable for at least 1 month Sus scrofa
0°C, loses approximately 25% of the activity during a 24-h period Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-glycolyl-neuraminate 9-phosphate + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Sus scrofa