Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Gold cells | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
C182A | no activity | Neisseria meningitidis |
C182S | no activity | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.6 | - |
N-acetyl-D-mannosamine | the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C | Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 271% relative activity compared to Mg2+ | Neisseria meningitidis | |
Mg2+ | 1 mM, required for 100% activity | Neisseria meningitidis | |
Mn2+ | 1 mM, 115% relative activity compared to Mg2+ | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38629 | - |
1 * 38629, calculated from amino acid sequence | Neisseria meningitidis |
38630 | - |
calculated from amino acid sequence | Neisseria meningitidis |
38649 | - |
1 * 38649, ESI-MS | Neisseria meningitidis |
38650 | - |
ESI-MS | Neisseria meningitidis |
39000 | - |
2 * 39000, SDS-PAGE | Neisseria meningitidis |
78000 | - |
SDS-PAGE | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Neisseria meningitidis | N-acetylneuraminate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE | Neisseria meningitidis |
monomer | 1 * 38629, calculated from amino acid sequence | Neisseria meningitidis |
monomer | 1 * 38649, ESI-MS | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
NeuNAc synthase | - |
Neisseria meningitidis |
sialic acid synthase | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Neisseria meningitidis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | - |
Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
N-acetyl-D-mannosamine | the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Neisseria meningitidis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Neisseria meningitidis |