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Literature summary for 2.5.1.56 extracted from

  • Hao, J.; Balagurumoorthy, P.; Sarilla, S.; Sundaramoorthy, M.
    Cloning, expression, and characterization of sialic acid synthases (2005), Biochem. Biophys. Res. Commun., 338, 1507-1514.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Gold cells Neisseria meningitidis

Protein Variants

Protein Variants Comment Organism
C182A no activity Neisseria meningitidis
C182S no activity Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.6
-
N-acetyl-D-mannosamine the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C Neisseria meningitidis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 271% relative activity compared to Mg2+ Neisseria meningitidis
Mg2+ 1 mM, required for 100% activity Neisseria meningitidis
Mn2+ 1 mM, 115% relative activity compared to Mg2+ Neisseria meningitidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38629
-
1 * 38629, calculated from amino acid sequence Neisseria meningitidis
38630
-
calculated from amino acid sequence Neisseria meningitidis
38649
-
1 * 38649, ESI-MS Neisseria meningitidis
38650
-
ESI-MS Neisseria meningitidis
39000
-
2 * 39000, SDS-PAGE Neisseria meningitidis
78000
-
SDS-PAGE Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Neisseria meningitidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Neisseria meningitidis N-acetylneuraminate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Neisseria meningitidis
monomer 1 * 38629, calculated from amino acid sequence Neisseria meningitidis
monomer 1 * 38649, ESI-MS Neisseria meningitidis

Synonyms

Synonyms Comment Organism
NeuNAc synthase
-
Neisseria meningitidis
sialic acid synthase
-
Neisseria meningitidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Neisseria meningitidis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70
-
Neisseria meningitidis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.83
-
N-acetyl-D-mannosamine the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C Neisseria meningitidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Neisseria meningitidis

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Neisseria meningitidis