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Literature summary for 2.5.1.55 extracted from

  • Nelson, S.K.; Kelleher, A.; Robinson, G.; Reiling, S.; Asojo, O.A.
    Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa (2013), Acta Crystallogr. Sect. F, 69, 1084-1088.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene kdo8ps, recombinant expression of His6-tagged codon-optimized enzyme in Escherichia coli strain Rosetta2 (pLysS) DE3 Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.003 ml of 5 mg/ml protein in 0.2 M Tris-HCl, pH 7.4, 0.1 M NaCl, 0.05% 2-mercaptoethanol, and 0.1 mM phosphoenolpyruvate with 0.0015 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 18% w/v PEG 8000, 20°C, 2 days, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacementusing structure PDB ID 1g7v and model building Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O Pseudomonas aeruginosa
-
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
gene kdo8ps
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2 (pLysS) DE3 by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
Pseudomonas aeruginosa 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
More two-dimensional structure comparison Pseudomonas aeruginosa
oligomer the enzyme structure is a prototypical alpha/beta TIM barrel structure expected from this family of enzymes and contains a tetramer in the asymmetric unit Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
2-dehydro-3-deoxyphosphooctonate aldolase
-
Pseudomonas aeruginosa
2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase
-
Pseudomonas aeruginosa
KDO8PS
-
Pseudomonas aeruginosa

General Information

General Information Comment Organism
additional information active site structure, overview Pseudomonas aeruginosa
physiological function the enzyme catalyzes a key step in the synthesis of the lipopolysaccharide of most Gram-negative bacteria, it catalyzes the condensation reaction between phosphoenolpyruvate and arabinose 5-phosphate to produce 3-deoxy-D-manno-octulosonate 8-phosphate. The enzyme is vital for for proper synthesis and assembly of lipoplysaccharides and the survival and virulence of Pseudomonas aeruginosa Pseudomonas aeruginosa