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Literature summary for 2.5.1.5 extracted from
- Purification, identification, and characterization of D-galactose-6-sulfurylase from marine algae (Betaphycus gelatinus) (2014), Carbohydr. Res., 388, 94-99.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Betaphycus gelatinus |  |
| Cu2+ | - |
Betaphycus gelatinus | |
| EDTA | - |
Betaphycus gelatinus | |
| Hg2+ | - |
Betaphycus gelatinus | |
| K+ | - |
Betaphycus gelatinus | |
| Mn2+ | - |
Betaphycus gelatinus | |
| Pb2+ | - |
Betaphycus gelatinus | |
| phenylmethylsulfonyl fluoride | - |
Betaphycus gelatinus | |
| SDS | - |
Betaphycus gelatinus | |
| Zn2+ | - |
Betaphycus gelatinus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activated by any metal ion | Betaphycus gelatinus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
1 * 65000, SDS-PAGE | Betaphycus gelatinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Betaphycus gelatinus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, HiTrap DEAE column chromatography, and phenyl column chromatography | Betaphycus gelatinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.03782 | - |
crude extract, at pH 7.0 and 40°C | Betaphycus gelatinus |
| 0.1865 | - |
after purification, at pH 7.0 and 40°C | Betaphycus gelatinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mu-carrageenan | - |
Betaphycus gelatinus | sulfate + kappa-carrageenan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Betaphycus gelatinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose-6-sulfurylase | - |
Betaphycus gelatinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Betaphycus gelatinus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | enzyme activity gradually increases in the temperature range from 10 to 40°C. The enzyme activity is less than half of the highest activity when the temperature is not more than 30°C and higher than 60°C. The enzyme activity is completely absent at 70°C | Betaphycus gelatinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Betaphycus gelatinus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | the activity at pH 6.5 and 9.5 is 23.6% and 0.9% of the maximum activity, respectively | Betaphycus gelatinus |
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Release 2023.1 (January 2023)
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