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Literature summary for 2.5.1.49 extracted from
- O-Acetylhomoserine sulfhydrylase of the fission yeast Schizosaccharomyces pombe: Partial purification, characterization, and its probable role in homocysteine biosynthesis (1984), J. Biochem., 96, 1511-1523.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Schizosaccharomyces pombe |  |
General Stability
| General Stability | Organism |
|---|---|
| very unstable, can be stabilized by addition of 25% sucrose or glycerol | Schizosaccharomyces pombe |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-Ethionine | - |
Schizosaccharomyces pombe | |
| Hydroxylamine hydrochloride | - |
Schizosaccharomyces pombe | |
| KCN | - |
Schizosaccharomyces pombe | |
| L-methionine | competitive inhibition | Schizosaccharomyces pombe | |
| L-Penicillamine | - |
Schizosaccharomyces pombe | |
| additional information | p-chloromercuribenzoic acid, 5,5'-dithio-bis(2-nitrobenzoic acid) and monoiodoacetic acid have no inhibitory effect | Schizosaccharomyces pombe | |
| O-acetyl-L-serine | - |
Schizosaccharomyces pombe | |
| O-phospho-L-serine | - |
Schizosaccharomyces pombe | |
| phenylhydrazine | - |
Schizosaccharomyces pombe | |
| S-adenosyl-L-homocysteine | - |
Schizosaccharomyces pombe | |
| S-adenosyl-L-methionine | - |
Schizosaccharomyces pombe | |
| Semicarbazide | - |
Schizosaccharomyces pombe | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
H2S | - |
Schizosaccharomyces pombe | |
| 10.4 | - |
L-homoserine | - |
Schizosaccharomyces pombe | |
| 11.1 | - |
O-succinyl-L-homoserine | - |
Schizosaccharomyces pombe | |
| 12.5 | - |
O-acetyl-L-homoserine | - |
Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
sucrose density gradient ultracentrifugation | Schizosaccharomyces pombe |
| 186000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-homoserine + H2S | Schizosaccharomyces pombe | - |
L-homocysteine + acetic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
fission yeast, IFO-0363 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizosaccharomyces pombe |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, can be stored without loss of catalytic activity | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-homoserine + H2S | - |
Schizosaccharomyces pombe | homocysteine + ? | - |
? | |
| additional information | does not synthesize cystathionine from O-acetyl-L-homoserine and L-cysteine, cannot utilize cysteine as co-substrate in place of H2S, O-acetyl-L-serine, O-acetyl-L-tyrosine, O-acetyl-L-threonine, O-phospho-DL-threonine, O-phospho-L-serine, L-serine-O-sulfate and O-acetyl-L-hydroxyproline are no substrates | Schizosaccharomyces pombe | ? | - |
? | |
| O-acetyl-L-homoserine + H2S | - |
Schizosaccharomyces pombe | L-homocysteine + acetic acid | - |
? | |
| O-acetyl-L-serine + H2S | - |
Schizosaccharomyces pombe | L-cysteine + acetic acid | - |
? | |
| O-succinyl-L-homoserine + H2S | - |
Schizosaccharomyces pombe | homocysteine + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Homocysteine synthase | - |
Schizosaccharomyces pombe |
| OAH sulfhydrylase | - |
Schizosaccharomyces pombe |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
more stable at 30°C than at 0°C | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Schizosaccharomyces pombe |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
optimal pH for stability | Schizosaccharomyces pombe |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Schizosaccharomyces pombe | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
L-methionine | - |
Schizosaccharomyces pombe | |
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