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Literature summary for 2.5.1.49 extracted from
- Methionine biosynthesis in Brevibacterium flavum: Properties and essential role of O-acetylhomoserine sulfhydrylase (1982), J. Biochem., 91, 1163-1171.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DL-cystathionine | 10 mM addition, AHS activity 105% | [Brevibacterium] flavum |  |
| pyridoxal 5'-phosphate | - |
[Brevibacterium] flavum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-homoserine | - |
[Brevibacterium] flavum | |
| L-methionine | - |
[Brevibacterium] flavum | |
| additional information | L-serine, glycine, cystathione and S-adenosyl-L-methionine are no inhibitors | [Brevibacterium] flavum | |
| O-acetyl-L-serine | - |
[Brevibacterium] flavum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
H2S | - |
[Brevibacterium] flavum | |
| 2 | - |
O-acetyl-L-homoserine | - |
[Brevibacterium] flavum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 360000 | - |
gel filtration | [Brevibacterium] flavum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-homoserine + H2S | [Brevibacterium] flavum | - |
L-homocysteine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methanethiol | [Brevibacterium] flavum | methionine biosynthetic pathway | L-methionine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Brevibacterium] flavum | - |
No. 2247, ATCC 14067 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Brevibacterium] flavum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00472 | - |
- |
[Brevibacterium] flavum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | shows with O-acetylserine 1% of the activity with O-acetylhomoserine, no activity with O-succinyl-L-homoserine, homoserine or serine | [Brevibacterium] flavum | ? | - |
? | |
| O-acetyl-L-homoserine + H2S | - |
[Brevibacterium] flavum | L-homocysteine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methanethiol | methionine biosynthetic pathway | [Brevibacterium] flavum | L-methionine + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AHS | - |
[Brevibacterium] flavum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
[Brevibacterium] flavum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
[Brevibacterium] flavum | |
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