General Stability | Organism |
---|---|
enzyme appears to be quite stable | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no end product inhibition by methionine or S-adenosylmethionine observed | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
H2S | - |
Neurospora crassa | |
0.8 | - |
methyl-mercaptan | - |
Neurospora crassa | |
7 | - |
O-acetyl-L-homoserine | - |
Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + H2S | Neurospora crassa | - |
L-homocysteine + acetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
-15°C, 80% of the activity remains after storage for 6 months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | L-homoserine, O-succinyl-DL-homoserine, O-phosphoryl-L-homoserine, L-serine, O-acetyl-L-serine, O-phosphoryl-L-serine react at less than 1% the rate with O-acetyl-L-homoserine | Neurospora crassa | ? | - |
? | |
O-acetyl-L-homoserine + H2S | - |
Neurospora crassa | L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-homoserine + methyl mercaptan | - |
Neurospora crassa | L-methionine + acetic acid | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | only required after enzyme is resolved by dialysis against cysteine | Neurospora crassa |