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Literature summary for 2.5.1.47 extracted from

  • Qabazard, B.; Ahmed, S.; Li, L.; Arlt, V.M.; Moore, P.K.; Stuerzenbaum, S.R.
    C. elegans aging is modulated by hydrogen sulfide and the sulfhydrylase/cysteine synthase cysl-2 (2013), PLoS ONE, 8, e80135.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene cysl-2, quantitative real-time PCR enzyme expression analysis Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Caenorhabditis elegans the enzyme shows H2S synthesizing activity, cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9 ?
-
 ?
O-acetyl-L-serine + hydrogen sulfide Caenorhabditis elegans
-
 L-cysteine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans O45679 gene cysl-2 or K10H10.2, and gene GYY4137
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows H2S synthesizing activity, cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9 Caenorhabditis elegans ?
-
 ?
O-acetyl-L-serine + hydrogen sulfide
-
 Caenorhabditis elegans L-cysteine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
CysK2
-
 Caenorhabditis elegans
CYSL
-
 Caenorhabditis elegans
cysl-2
-
 Caenorhabditis elegans
GYY4137
-
 Caenorhabditis elegans
sulfhydrylase/cysteine synthase
-
 Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Caenorhabditis elegans

General Information

General Information Comment Organism
malfunction cysl-2 mutant phenotype, overview. The cysl-2 mutant has a reduced rate of enzymatic H2S synthesis from the added substrates L-cysteine and is marked by a decline in reproductive output compared to wild-type worms. Body area and length are significantly lower in cysl-2 compared to age-matched wild type worms, and GYY4137 treatment effectively rescues this phenotype. Lifespan is reduced in cysl-2 compared to wild-type and the addition of GYY4137 reverses this effect and increases the median survival of cysl-2 Caenorhabditis elegans
physiological function the bifunctional enzyme, encoded by gene K10H10.2, or cysl-2, shows cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9. It has been described as a hypoxia inducible factor (HIF) target. The enzyme regulates the mRNA expression of aging-associated and stress-related genes in wild-type Caenorhabditis elegans. Enzyme treatment protects the wild-type nematodes against oxidative, endoplasmic reticulum stress, and thermal stressors but not cadmium-induced metallic stress. The enzyme is active in vitro and in vivo and protects against lipopolysaccharide-induced inflammation. GYY4137 induces longevity and stress resistance, mechanism of action via induction of T24B8.5 and altered transcriptional control of SKN-1 Caenorhabditis elegans