Literature summary for 2.5.1.47 extracted from

Bogicevic, B.; Berthoud, H.; Portmann, R.; Meile, L.; Irmler, S.
CysK from Lactobacillus casei encodes a protein with O-acetylserine sulfhydrylase and cysteine desulfurization activity (2012), Appl. Microbiol. Biotechnol., 94, 1209-1220.

Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Lacticaseibacillus casei

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	presence of 4% NaCl is not inhibitory	Lacticaseibacillus casei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	-	O-acetyl-L-Ser	pH 7.4, 37°C	Lacticaseibacillus casei
0.7	-	dithiothreitol	pH 7.4, 37°C	Lacticaseibacillus casei
0.7	-	L-Cys	pH 7.4, 37°C	Lacticaseibacillus casei
6.7	-	H2S	pH 7.4, 37°C	Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	PAGE	Lacticaseibacillus casei
34900	-	1 * 32000, SDS-PAGE, 1 * 34900, calculated	Lacticaseibacillus casei

Organism

Organism	UniProt	Comment	Textmining
Lacticaseibacillus casei	E5LPF4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Cys + dithiothreitol	-	Lacticaseibacillus casei	beta-cyanoalanine + H2S	-	?
additional information	no substrates: serine, phosphoserine, O-succinylhomoserine, thiosulfate	Lacticaseibacillus casei	?	-	?
O-acetyl-L-Ser + H2S	-	Lacticaseibacillus casei	L-Cys + acetate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 32000, SDS-PAGE, 1 * 34900, calculated	Lacticaseibacillus casei

Synonyms

Synonyms	Comment	Organism
cysK	-	Lacticaseibacillus casei

Expression

Organism	Comment	Expression
Lacticaseibacillus casei	expression is not regulated by presence of cysteine	additional information

General Information

General Information	Comment	Organism
physiological function	enzyme is able to complement the cysteine auxotrophy of an Escherichia coli cysMK mutant	Lacticaseibacillus casei

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Release 2023.1 (January 2023)