Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces lavendulae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
hydrogen sulfide | 30°C, pH 8.0 | Streptomyces lavendulae | |
22 | - |
thiosulfate | 30°C, pH 8.0 | Streptomyces lavendulae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Streptomyces lavendulae |
126000 | - |
gel filtration | Streptomyces lavendulae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | D2Z027 | - |
- |
Streptomyces lavendulae ATCC 11924 | D2Z027 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency | Streptomyces lavendulae | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency | Streptomyces lavendulae ATCC 11924 | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + L-homocysteine | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae | cystathionine + acetate | - |
? | |
O-acetyl-L-serine + L-homocysteine | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae ATCC 11924 | cystathionine + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae | S-sulfo-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae ATCC 11924 | S-sulfo-L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Streptomyces lavendulae |
Synonyms | Comment | Organism |
---|---|---|
DcsD | - |
Streptomyces lavendulae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces lavendulae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.72 | - |
thiosulfate | 30°C, pH 8.0 | Streptomyces lavendulae | |
12 | - |
hydrogen sulfide | 30°C, pH 8.0 | Streptomyces lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces lavendulae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
thiosulfate | 30°C, pH 8.0 | Streptomyces lavendulae | |
0.14 | - |
O-acetyl-L-serine | 30°C, pH 8.0, cosubstrate: hydrogen sulfide | Streptomyces lavendulae | |
0.19 | - |
L-homocysteine | 30°C, pH 8.0 | Streptomyces lavendulae | |
100 | - |
hydrogen sulfide | 30°C, pH 8.0 | Streptomyces lavendulae |