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Literature summary for 2.5.1.47 extracted from

  • Kumaran, S.; Yi, H.; Krishnan, H.B.; Jez, J.M.
    Assembly of the cysteine synthase complex and the regulatory role of protein-protein interactions (2009), J. Biol. Chem., 284, 10268-10275.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67600
-
 calculated from cDNA Glycine max
70000
-
 gel filtration Glycine max
74200
-
 sedimentation equilibrium ultracentrifugation Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max Q8W1A0
-
-

Purification (Commentary)

Purification (Comment) Organism
purified using nickel-affinity and gel filtration. Thrombin digestion is used to remove the His tag from each protein Glycine max

Subunits

Subunits Comment Organism
homodimer gel filtration Glycine max
More a cysteine synthase complex is formed by association of serine O-acetyltransferase (SAT) and O-acetylserine sulfhydrylase (OASS). Biophysical examination cysteine synthase complex by gel filtration and sedimentation ultracentrifugation indicates that this assembly consists of a single serine O-trimer and three OASS dimers Glycine max

Synonyms

Synonyms Comment Organism
O-acetylserine sulfhydrylase
-
 Glycine max
OASS
-
 Glycine max