Application | Comment | Organism |
---|---|---|
medicine | humans lack cysteine synthase. Therefore, this parasite enzyme could be an exploitable drug target | Trichomonas vaginalis |
Cloned (Comment) | Organism |
---|---|
- |
Trichomonas vaginalis |
Protein Variants | Comment | Organism |
---|---|---|
K214A | mutant retains high activity with O-acetylserine and sulfide (40% of the activity of the wild type enzyme), but its activity with O-phosphoserine and sulfide is reduced by more than 100fold. The ability to use thiosulfate as an alternative nucleophile in the sulfhydrylase reaction is greatly reduced, but the mutant shows no change in cysteine desulfurase activity | Trichomonas vaginalis |
K43A | protein is yellow, indicating that it binds pyridoxal 5'-phosphate but has no detectable activity as a cysteine synthase with O-acetylserine or O-phosphoserine and no detectable cysteine desulfurase activity | Trichomonas vaginalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
H2S | 37°C, pH 7.8, cosubstrate: O-phosphoserine | Trichomonas vaginalis | |
0.8 | - |
L-Cys | 37°C, pH 7.8 | Trichomonas vaginalis | |
0.8 | - |
H2S | 37°C, pH 7.8, cosubstrate: O-acetyl-L-Ser | Trichomonas vaginalis | |
39.5 | - |
O-acetyl-L-Ser | 37°C, pH 7.8 | Trichomonas vaginalis | |
227 | - |
O-phosphoserine | 37°C, pH 7.8 | Trichomonas vaginalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichomonas vaginalis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
98.5 | - |
reaction with O-phosphoserine | Trichomonas vaginalis |
180 | - |
reaction with O-acetyl-L-Ser | Trichomonas vaginalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-Ser + H2S | - |
Trichomonas vaginalis | L-Cys + acetate | - |
r | |
O-acetyl-L-serine | - |
Trichomonas vaginalis | L-cysteine + acetate | - |
? | |
O-phosphoserine + H2S | - |
Trichomonas vaginalis | L-Cys + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TvCS1 | - |
Trichomonas vaginalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
L-Cys | 37°C, pH 7.8 | Trichomonas vaginalis | |
153 | - |
O-acetyl-L-Ser | 37°C, pH 7.8 | Trichomonas vaginalis | |
165 | - |
O-phosphoserine | 37°C, pH 7.8 | Trichomonas vaginalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Trichomonas vaginalis |